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MABN328 Anti-peptidyl-citrulline, clone F95 Antibody

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MABN328
100 µg  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, Ch, RIHC, WB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN328
DescriptionAnti-peptidyl-citrulline, clone F95 Antibody
Alternate Names
  • Peptidyl-citrulline
  • Protein-bound citrulline
Background InformationDeimination (citrullination) is a post translational modification in which the peptidyl-arginine deiminase family of enzymes convert the guanidino side chain of the amino acid arginine into a ureido group, yeilding peptidyl-citrulline. As a result, anti-peptidyl-citrulline antibodies should also cross react with peptidyl-homocitrulline, produced by the carbamylation of lysine, but not free citrulline, which is created in the urea cycle, or as a by-product of nitric oxide synthesis.
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-peptidyl-citrulline, clone F95 Antibody is validated for use in Immunohistochemistry and Western Blotting and Immunocytochemistry for the detection of peptidyl-citrulline.
Key Applications
  • Immunohistochemistry
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected peptidyl-citrulline in human cerebellum, and rat cerebellum tissue.
Western Blotting Analysis: A representative lot detected peptidyl-citrulline in CNS proteins from brains and spinal cord of EAE mice (Nicholas et al., 2005).
Biological Information
ImmunogenCitrullinated peptide consisting of 10 citrulline residues and a carboxyl Gly-Gly-Cys.
CloneF95
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgMκ
Species Reactivity
  • Human
  • Mouse
  • Chicken
  • Rat
Species Reactivity NotePredicted to react with All based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Molecular WeightVarious kDa calculated
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human cerebral cortex tissue.

Imunnohistochemistry Analysis: A 1:250 dilution of this antibody detected peptidyl-citrulline inhuman cerebral cortex tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MABN328 04053252996184