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07-227-I-100UG Anti-p34-Arc/ARPC2

Anti-p34-Arc/ARPC2 , Cat. No. 07-227-I, is a rabbit polyclonal antibody that detects Actin-related protein 2/3 complex subunit 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

07-227-I-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number07-227-I-100UG
DescriptionAnti-p34-Arc/ARPC2
Alternate Names
  • Actin-related protein 2/3 complex subunit 2
  • Arp2/3 complex 34 kDa subunit
  • p34-ARC
Background InformationActin-related protein 2/3 complex subunit 2 (UniProt: O15144; also known as Arp2/3 complex 34 kDa subunit, p34-ARC, ARPC2) is encoded by the ARPC2 (also known as ARC34, PRO2446) gene (Gene ID: 10109) in human. ARPC2 is an evolutionarily conserved actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). It holds a central structural position and helps relay both signals and conformational changes. ARPC2 also participates in promoting smooth muscle cell migration. The Arp2/3 complex is shown to mediate the formation of branched actin networks in the cytoplasm, which provides the force for cell motility. This complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription. It is also involved in promoting homologous recombination repair in response to DNA damage. ARPC2 is shown to increase the proliferation and invasion in MKN-28, human gastric cancer, cells. The expression of ARPC2 has been linked with aggressiveness gastric cancer that includes large tumor size, lymph node invasion, high tumor stage, and poor prognosis. (Ref.: Zhang, J., et al. (2017). Mediators of Inflammation. 217; Article ID 5432818).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-p34-Arc/ARPC2 , Cat. No. 07-227-I, is a rabbit polyclonal antibody that detects Actin-related protein 2/3 complex subunit 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected p34-Arc/ARPC2 in HeLa, HEK293, and Jurkat cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p34-Arc/ARPC2 in HeLa cell line.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected p34-Arc/ARPC2 in human colon cancer and human tonsil tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Actin-related protein 2/3 complex subunit 2 (p34-ARC).
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityAnti-Anti-p34-Arc\ARPC2 is a rabbit polyclonal antibody that detects Actin-related protein 2/3 complex subunit 2. It targets an epitope with in 14 amino acids from the C-terminal region.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Mouse, and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ARPC2
  • ARC34
  • PRO2446
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~34 kDa observed; 34.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected p34-Arc/ARPC2 in A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-227-I-100UG 04054839007163