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MABT121 Anti-Vimentin Antibody, clone 3CB2

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MABT121
100 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Ch, R, LzWB, ICC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT121
DescriptionAnti-Vimentin Antibody, clone 3CB2
Alternate Names
  • Vimentin
Background InformationVimentin is a class III intermediate filament protein (homopolymers) found in various non-epithelial cells including mesenchymal cells and fibroblasts. In many cells of mesenchymal origin, it is one of the most prominent phosphoproteins, especially during cell division when enhanced phosphorylation of vimentin corresponds with the reorganization of vimentin filaments.
References
Product Information
FormatPurified
Control
  • ARPE cell lysate
PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Vimentin Antibody, clone 3CB2 is an antibody against Vimentin for use in WB, IC, IH.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunocytochemistry Analysis: A representative lot was used by an independent laboratory in human central and peripheral retinal sections (Perez-Alvarez, M.J., et al. (2008). J Neurosci Res. 86(8):1871-1883).

Western Blot Analysis:
A representative lot was used to detect vimentin in chicken and rat total brain extracts (Prada, FA., et al. (1995). GLIA 15:389-400).

Immunohistochemistry Analysis:
A representative lot was used to detect vimentin in E3 chick embryo sections and different regions of the developing chick central nervous system (Prada, FA., et al. (1995). GLIA 15:389-400).
Biological Information
ImmunogenVimentin extracted from chicken embryonic brain tissue.
Clone3CB2
HostMouse
IsotypeIgMκ
Species Reactivity
  • Human
  • Chicken
  • Rat
  • Lizard
Species Reactivity NoteDemonstrated to react with Human.
Demonstrated to react with Chameleon and Rat based on the following reference:
GLIA 15:389-400 (1995), Prada, F. A., et. al.
Predicted to react to Chicken based on immunogen design.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • VIM
UniProt Number
UniProt SummaryFUNCTION: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

SUBUNIT STRUCTURE: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM.
Molecular Weight~54 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in ARPE19 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Vimentin in ARPE19 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT121 04053252293795

Documentation

Anti-Vimentin Antibody, clone 3CB2 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Vimentin Antibody, clone 3CB2 Certificates of Analysis

TitleLot Number
Anti-Vimentin, Clone 3CB2 - 4142003 4142003
Anti-Vimentin, clone 3CB2 2839589
Anti-Vimentin, clone 3CB2 - 2540028 2540028
Anti-Vimentin, clone 3CB2 - 2562386 2562386
Anti-Vimentin, clone 3CB2 - 3168750 3168750
Anti-Vimentin, clone 3CB2 - 3225899 3225899
Anti-Vimentin, clone 3CB2 - 3574548 3574548
Anti-Vimentin, clone 3CB2 - 3931718 3931718
Anti-Vimentin, clone 3CB2 - NRG1906477 NRG1906477
Anti-Vimentin, clone 3CB2 -2592714 2592714

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Product Families

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies