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DR1047 Anti-UHRF1 Mouse mAb (3A11)

This Anti-UHRF1 Mouse mAb (3A11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of UHRF1.

Anti-UHRF1 Mouse mAb (3A11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Ubiquitin-Like PHD/RING Finger Domain 1

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DR1047
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
DR1047-100UGCN 100 μg
Description
OverviewRecognizes the ~90 kDa UHRF1 protein in HeLa cells and human endometrium tissue.
Catalogue NumberDR1047
Brand Family Calbiochem®
SynonymsAnti-Ubiquitin-Like PHD/RING Finger Domain 1
Application Data
Detection of human UHRF1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human UHRF1 by staining paraffin sections. Sample: Formalin-fixed, paraffin embedded human endometrium tissue. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (3 µg/ml). Detection: colorimetric (DAB).

Detection of human UHRF1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (10 µg/ml). Detection: fluorescence.
References
ReferencesMistry H, et al. 2008. Biochem. Biophys. Res. Commun. 375 321.
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells, Human endometrium tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acides 694-794 of human rUHRF1, expressed as a GST fusion protein
ImmunogenNA
Clone3A11
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1047-100UGCN 04055977225969

Documentation

Anti-UHRF1 Mouse mAb (3A11) MSDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Mistry H, et al. 2008. Biochem. Biophys. Res. Commun. 375 321.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-November-2010 RFH
SynonymsAnti-Ubiquitin-Like PHD/RING Finger Domain 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human UHRF1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human UHRF1 by staining paraffin sections. Sample: Formalin-fixed, paraffin embedded human endometrium tissue. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (3 µg/ml). Detection: colorimetric (DAB).

Detection of human UHRF1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~90 kDa UHRF1 protein.
BackgroundThis protein is a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIα and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint
HostMouse
Immunogen speciesNA
ImmunogenA recombinant polypeptide corresponding to amino acides 694-794 of human rUHRF1, expressed as a GST fusion protein
Clone3A11
IsotypeIgG₁
Specieshuman
Positive controlHeLa cells, Human endometrium tissue
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesMistry H, et al. 2008. Biochem. Biophys. Res. Commun. 375 321.