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05-661-I-100UG Anti-β-Tubulin Antibody, clone AA2

Anti-β-Tubulin, clone AA2, Cat. No. 05-661-I, is a mouse monoclonal antibody that detects β-Tubulin and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

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05-661-I-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue Number05-661-I-100UG
DescriptionAnti-β-Tubulin Antibody, clone AA2
Alternate Names
  • Tubulin beta-5 chain
  • beta-Tubulin
Background InformationTubulin β chain (UniProt: Q2KJD0; also known as Tubulin beta-5 chain) is encoded by the TUBB gene (Gene ID: 615087) in human. Tubulin is the major constituent of microtubules (MTs). MTs are cytoskeletal filaments composed ofa/β-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. a/β heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the β-tubulin subunit facing the microtubule plus end conferring a structural polarity. Tubulin binds two moles of GTP, one at an exchangeable site on the β-chain and one at a non-exchangeable site on the a-chain. Tubulin dimers constantly polymerize and depolymerize, and MTs can undergo rapid cycles of assembly and disassembly. The first stage of MT formation, the nucleation phase, is slow. In the presence of Mg2+ and GTP, a and β tubulins join in an end-to-end manner to form protofilaments with alternating a and β subunits. GTP bound to β-tubulin is hydrolyzed to GDP during or immediately after polymerization. This weakens the binding affinity of tubulin for adjacent molecules and favors depolymerization that contributes to the dynamic behavior of MTs. MTs also undergo treadmilling, in which tubulin molecules bound to GDP are continually lost from the minus end and are replaced by the addition of GTP-bound tubulin molecules to the plus end of the same MT. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins and motor proteins. β-Tubulin is reported to undergo phosphorylation on serine 172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-β-Tubulin, clone AA2, Cat. No. 05-661-I, is a mouse monoclonal antibody that detects β-Tubulin and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunofluorescence
Application NotesTested Applications

Immunofluorescence Analysis: A representative lot detected β-Tubulin in Immunofluorescence applications (Abdelfattah, N., et al. (2018). Nat Commun. 9(1):4541; Kelly, J.J., et al. (2015). J Cell Sci. 128(21):3947-60; Moreira, B.P., et al. (2017). J Cell Sci. 130(6):1179-1193; Verbist, K.C., et al. (2016). Nature. 532(7599):389-93).

Immunocytochemistry Analysis: A representative lot detected β-Tubulin in Immunocytochemistry applications (Verbist, K.C., et al. (2016). Nature. 532(7599):389-93).

Western Blotting Analysis: A representative lot detected β-Tubulin in Western Blotting applications (Abdelfattah, N., et al. (2018). Nat Commun.;9(1):4541; Cho, J-H., et al. (2017). Nat Commun. 8(1):1848; Moreira, B.P., et al. (2017). J Cell Sci. 130(6):1179-1193; Ohshiro, K., et al. (2020). Genes Cancer. 11(1-2):43-52; Rawlinson, S.M., et al. (2018). Nat Commun. 9(1):3057; Shrestha, P., et al. (2020). Nat Neurosci. 23(2):281-292; Terzaghi, L., et al. (2018). Reproduction. 155(3):273-282; Villar-Fincheira, P., et al. (2021). Front Physiol.12:722528).

Immunohistochemistry Applications: A representative lot detected β-Tubulin in Immunohistochemistry applications (Cho, J-H., et al. (2017). Nat Commun. 8(1):1848).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenPurified β-tubulin from bovine brain.
EpitopeC-terminal
CloneAA2
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone AA2 is a mouse monoclonal antibody that detects β-Tubulin. It targets an epitope within 19 amino acids from the C-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Bovine
  • Rabbit
  • Rat
  • Mouse
  • Syrian Hamster
  • Human
Species Reactivity NoteHuman, Bovine, Hamster, Mouse, Rat, Rabbit.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TUBB5
Purification MethodProtein G purified
UniProt Number
Molecular Weight~50 kDa observed; 49.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected β-Tubulin in A431 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-661-I-100UG 04065267061634

Documentation

Anti-β-Tubulin Antibody, clone AA2 MSDS

Title

Safety Data Sheet (SDS) 

Anti-β-Tubulin Antibody, clone AA2 Certificates of Analysis

TitleLot Number
Anti-β-Tubulin, clone AA2 - 3956067 3956067
Anti-β-Tubulin, clone AA2 - 3981709 3981709
Anti-β-Tubulin, clone AA2 - 4010119 4010119
Anti-β-Tubulin, clone AA2 - 4010718 4010718
Anti-β-Tubulin, clone AA2 - 4014162 4014162
Anti-β-Tubulin, clone AA2 - 4023767 4023767
Anti-β-Tubulin, clone AA2 - 4036268 4036268
Anti-β-Tubulin, clone AA2 - 4046621 4046621
Anti-β-Tubulin, clone AA2 - 4048037 4048037
Anti-β-Tubulin, clone AA2 - 4049876 4049876
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