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AB9913 Anti-TNRC6B Antibody

Anti-TNRC6B Antibody is a Rabbit Polyclonal Antibody for detection of TNRC6B also known as Trinucleotide repeat-containing gene 6B protein & has been validated in WB, ICC & IP.

Anti-TNRC6B Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: TNRC6B, KIAA1093 UniProt Number: Q9UPQ9
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AB9913
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, OxWB, ICC, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB9913
DescriptionAnti-TNRC6B Antibody
Alternate Names
  • Trinucleotide repeat-containing gene 6B protein
Background InformationTNRC6B (trinucleotide repeat containing 6B) is a argonaute-associated protein that localizes to cytoplasmic P bodies and is essential for control of miRNA-guided mRNA cleavage. TNRC6B is one of the mammalian homologs of the metazoan GW182 and is required for well-organized miRNA repression. Studies suggest that TNRC6B-mediated miRNA silencing is promoted by the C-terminal silencing domain.
References
Product Information
FormatAffinity Purified
Control
  • HeLa cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TNRC6B Antibody is a Rabbit Polyclonal Antibody for detection of TNRC6B also known as Trinucleotide repeat-containing gene 6B protein & has been validated in WB, ICC & IP.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected TNRC6B in HeLa cells. (Zipprich, J., et al. (2009). RNA. 15:781–793.)
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human TNRC6B.
EpitopeN-terminan domain of TNRC6B
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityAntibody recognizes TNRC6B
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Ox
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TNRC6B
  • KIAA1093
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent translational repression and siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. Ref.7 Ref.11 Ref.12

SUBUNIT STRUCTURE: Interacts with EIF2C1/AGO1, EIF2C2/AGO2, EIF2C3/AGO3 and EIF2C4/AGO4. Ref.7 Ref.11 Ref.12 Ref.9

SUBCELLULAR LOCATION: Cytoplasm › P-body. Note: Mammalian P-bodies are also known as GW bodies (GWBs). Ref.7 Ref.11

SEQUENCE SIMILARITIES: Belongs to the GW182 family.

Contains 1 RRM (RNA recognition motif) domain.

SEQUENCE CAUTION: The sequence BAA83045.2 differs from that shown. Reason: Erroneous initiation.
Molecular Weight~150/190 kDa observed. (Meister, G., et al. (2005). Current Biology. 15:2149–2155). An uncharacterized band at ~39 kDa may be observed in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected TNRC6B on 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB9913 04053252685071

Documentation

Anti-TNRC6B Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-TNRC6B Antibody Certificates of Analysis

TitleLot Number
Anti-TNRC6B - 2142186 2142186
Anti-TNRC6B - 2379500 2379500
Anti-TNRC6B - 2273842 2273842
Anti-TNRC6B - 3188704 3188704
Anti-TNRC6B - 3429918 3429918
Anti-TNRC6B - 3735395 3735395
Anti-TNRC6B - 3894622 3894622
Anti-TNRC6B - 4052389 4052389
Anti-TNRC6B - 4140612 4140612
Anti-TNRC6B - NRG1887615 NRG1887615
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Brochure

Title
New Products: Volume 3, 2012