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AB5535-AF555 Anti-Sox9 Antibody, Alexa Fluor® 555 conjugate

This Anti-Sox9 Antibody, Alexa Fluor® 555 conjugate is validated for use in Immunocytochemistry for the detection of Sox9.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: SOX9 UniProt Number: P48436
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AB5535-AF555
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, H, MICCRbAlexaFluor®555Polyclonal Antibody
Description
Catalogue NumberAB5535-AF555
DescriptionAnti-Sox9 Antibody, Alexa Fluor® 555 conjugate
Alternate Names
  • Transcription factor SOX-9
  • Sox9
Background InformationTranscription factor SOX-9 (UniProt P48436; also known as Sex-determining region Y-box 9 protein, SRY-related HMG-box gene 9) is encoded by the SOX9 (also known as CMD1, CMPD1, SRA1) gene (Gene ID 6662) in human. Sox family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences that encode for the HMG-box domains that mediate sequence-specific DNA binding. Sox genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at at least 40 genomic loci and at least 30 Sox genes have been identified.
References
Product Information
FormatAlexaFluor®555
PresentationPurified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Sox9 Antibody, Alexa Fluor® 555 conjugate is validated for use in Immunocytochemistry for the detection of Sox9.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sox9 in HepG2 cells.
The unconjugated version (Cat. No. AB5535) is also shown to be suitable for Western blotting and immunohistochemistry applications.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Sox9.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Rat
  • Human
  • Mouse
Species Reactivity NoteRat, Human, Mouse. Predicted to react with Horse, Bovine, Feline, Sheep based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SOX9
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight55-65 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Sox9 in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5535-AF555 04055977277197

Documentation

Anti-Sox9 Antibody, Alexa Fluor® 555 conjugate MSDS

Title

Safety Data Sheet (SDS) 

Anti-Sox9 Antibody, Alexa Fluor® 555 conjugate Certificates of Analysis

TitleLot Number
Anti-Sox9, Alexa Fluor® 555 conjugate 2929104
Anti-Sox9, Alexa Fluor® 555 conjugate - 3195392 3195392
Anti-Sox9, Alexa Fluor® 555 conjugate - 3232333 3232333
Anti-Sox9, Alexa Fluor® 555 conjugate - 3310656 3310656
Anti-Sox9, Alexa Fluor® 555 conjugate - 3321806 3321806
Anti-Sox9, Alexa Fluor® 555 conjugate - 3843665 3843665
Anti-Sox9, Alexa Fluor® 555 conjugate - 3934953 3934953
Anti-Sox9, Alexa Fluor® 555 conjugate - 4134651 4134651
Anti-Sox9, Alexa Fluor® 555 conjugate - 4177872 4177872
Anti-Sox9, Alexa Fluor® 555 conjugate -Q2595868 Q2595868

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.