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ABE30 Anti-RNA polymerase II subunit B1 Antibody

Use Anti-RNA polymerase II subunit B1 Antibody, rabbit polyclonal antibody validated in WB, ICC to detect RNA polymerase II subunit B1 also known as DNA-directed RNA polymerase II subunit RPB1.

Anti-RNA polymerase II subunit B1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: RpIILS, POLRA, RPB1, hsRPB1, RPBh1, RPO2, POLR2, hRPB220, RPOL2 UniProt Number: P24928
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ABE30
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, B, Eq, CaWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE30
DescriptionAnti-RNA polymerase II subunit B1 Antibody
Alternate Names
  • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • DNA-directed RNA polymerase III largest subunit
  • DNA-directed RNA polymerase II subunit RPB1
  • RNA polymerase II subunit B1
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit
  • RNA-directed RNA polymerase II subunit RPB1
  • polymerase (RNA) II (DNA directed) polypeptide A (220kD)
Background InformationRNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RPB1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing.
References
Product Information
FormatAffinity Purified
Control
  • HeLa nuclear extract
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-RNA polymerase II subunit B1 Antibody, rabbit polyclonal antibody validated in WB, ICC to detect RNA polymerase II subunit B1 also known as DNA-directed RNA polymerase II subunit RPB1.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected RNA polymerase II subunit B1 in HeLa cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human RNA polymerase II subunit B1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Bovine
  • Horse
  • Canine
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Horse, and Canine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummarySummary: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq].
Gene Symbol
  • RpIILS
  • POLRA
  • RPB1
  • hsRPB1
  • RPBh1
  • RPO2
  • POLR2
  • hRPB220
  • RPOL2
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.

CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

SUBUNIT STRUCTURE: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. The phosphorylated C-terminal domain interacts with FNBP3 and SYNCRIP. Interacts with SAFB/SAFB1. Interacts with CCNL1 and MYO1C By similarity. Interacts with CCNL2 and SFRS19. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with PAF1. Interacts (via C-terminus) with FTSJD2, CTDSP1 and RBM16. Interacts via the phosphorylated C-terminal domain with WDR82 and with SETD1A and SETD1B only in the presence of WDR82.

SUBCELLULAR LOCATION: Nucleus.

POST-TRANSLATIONAL MODIFICATION: The tandem 7 residues repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapepdtide repeat. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphataes, and a "CTD code" that specifies the position of Pol II within the transcription cycle has been proposed. Dephosphorylated by the protein phosphatase CTDSP1.

Ubiquitinated by WWP2 leading to proteasomal degradation (by similarity).

MISCELLANEOUS: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion temporarily coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits. The ribonucleoside triphosphate is transferred by a rotation to the nucelotide addition (A) site for pairing with the template DNA. The catalytic A site involves three conserved aspartate residues of the RNA Pol II largest subunit which permanently coordinate a second magnesium ion.

SEQUENCE SIMILARITIES: Belongs to the RNA polymerase beta' chain family.
Molecular Weight~217 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1 µg/mL of this antibody detected RNA polymerase II subunit B1 on 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE30 04053252750892

Documentation

Anti-RNA polymerase II subunit B1 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-RNA polymerase II subunit B1 Antibody Certificates of Analysis

TitleLot Number
Anti-RNA polymerase II subunit B1 3076899
Anti-RNA polymerase II subunit B1 (rabbit polyclonal) Polyclonal Antibody Q2909964
Anti-RNA polymerase II subunit B1 - 2427515 2427515
Anti-RNA polymerase II subunit B1 - 2026876 2026876
Anti-RNA polymerase II subunit B1 - 3823696 3823696
Anti-RNA polymerase II subunit B1 - NRG1868647 NRG1868647
Anti-RNA polymerase II subunit B1 -2816550 2816550
Anti-RNA polymerase II subunit B1 Polyclonal Antibody 2872939

References

Reference overviewPub Med ID
Reduced RAN expression and disrupted transport between cytoplasm and nucleus; a key event in Alzheimer's disease pathophysiology.
Mastroeni, D; Chouliaras, L; Grover, A; Liang, WS; Hauns, K; Rogers, J; Coleman, PD
PloS one  8  e53349  2013

Show Abstract 		23308199 23308199

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies