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MABE341 Sigma-AldrichAnti-Puromycin Antibody, clone 17H1

Anti-Puromycin antibody, clone 17H1 detects puromycin incorporated into protein. Monoclonal antibodies to puromycin may also be used with standard immunochemical methods.

Anti-Puromycin Antibody, clone 17H1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, A	WB	R	Purified	Monoclonal Antibody

Description
Catalogue Number	MABE341
Description	Anti-Puromycin Antibody, clone 17H1
Background Information	Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.

References

Product Information
Format	Purified
HS Code	3002 15 90
Control	HEK293 cell lysates treated with Puromycin and Cyclohexamide, or with Puromycin only.
Presentation	Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Puromycin antibody, clone 17H1 detects puromycin incorporated into protein. Monoclonal antibodies to puromycin may also be used with standard immunochemical methods.
Key Applications	Western Blotting

Biological Information
Immunogen	Puromycin from Streptomyces alboniger
Clone	17H1
Host	Rat
Isotype	IgG2aκ
Species Reactivity	Human All
Species Reactivity Note	Demonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
Antibody Type	Monoclonal Antibody
Purification Method	Protein G Purified
Molecular Weight	Puromycin is incorporated in neosynthesized proteins. In the presense of Puromycin only, this antibody detects Puromycin-incorporated neosynthesized proteins at multiple molecular weights. However, a decrease in signal is observed in the co-presense of Cycloheximide, an inhibitor of protein biosynthesis in eukaryotic organisms.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HEK293 cell lysates treated with Puromycin and Cyclohexamide, or with Puromycin only. Western Blotting Analysis: A 1:100,000 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HEK293 cell lysates treated with Puromycin only. This antibody also detected small mounts of Puromycin-incorporated neosynthesized proteins in HEK293 cells treated with Puromycin and Cyclohexamide.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting in HEK293 cell lysates treated with Puromycin and Cyclohexamide, or with Puromycin only.

Western Blotting Analysis: A 1:100,000 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HEK293 cell lysates treated with Puromycin only. This antibody also detected small mounts of Puromycin-incorporated neosynthesized proteins in HEK293 cells treated with Puromycin and Cyclohexamide.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
MABE341	04053252909214

Documentation

Anti-Puromycin Antibody, clone 17H1 MSDS

Title
Safety Data Sheet (SDS)

Anti-Puromycin Antibody, clone 17H1 Certificates of Analysis

Title	Lot Number
Anti-Puromycin, clone 17H1	3082497
Anti-Puromycin, clone 17H1 - 3194622	3194622
Anti-Puromycin, clone 17H1 - 3258755	3258755
Anti-Puromycin, clone 17H1 - 3403489	3403489
Anti-Puromycin, clone 17H1 - 3600708	3600708
Anti-Puromycin, clone 17H1 - 3808797	3808797
Anti-Puromycin, clone 17H1 - 3928038	3928038
Anti-Puromycin, clone 17H1 - 3948852	3948852
Anti-Puromycin, clone 17H1 - 4093161	4093161
Anti-Puromycin, clone 17H1 - Q2192701	Q2192701

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