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16-204 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate validated for use in WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: General Post-translation Modification
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16-204
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWBMBiotinMonoclonal Antibody
Description
Catalogue Number16-204
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate
OverviewIncluded Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate

Product Description: Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
Background InformationProduced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
Product Information
FormatBiotin
Control
  • A431 carcinoma cells.
    Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting.
PresentationBiotin-conjugated recombinant mouse IgG2bκ in PBS containing 0.05% sodium azide. Liquid at 2-8°C.
Quality LevelMQ100
Applications
ApplicationDetect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenPhosphotyramine-KLH
EpitopeN/A
Clone4G10®
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityTyrosine-phosphorylated proteins from all species.
IsotypeIgG2bκ
Species Reactivity
  • All
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein G Purified
Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot to detect tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 9 months at 2-8ºC from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. DO NOT FREEZE!
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
16-204 04053252366734