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16-204 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate validated for use in WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: General Post-translation Modification
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16-204
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWBMBiotinMonoclonal Antibody
Description
Catalogue Number16-204
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate
OverviewIncluded Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate

Product Description: Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
Background InformationProduced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
References
Product Information
FormatBiotin
Control
  • A431 carcinoma cells.
    Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting.
PresentationBiotin-conjugated recombinant mouse IgG2bκ in PBS containing 0.05% sodium azide. Liquid at 2-8°C.
Quality LevelMQ100
Applications
ApplicationDetect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenPhosphotyramine-KLH
EpitopeN/A
Clone4G10®
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityTyrosine-phosphorylated proteins from all species.
IsotypeIgG2bκ
Species Reactivity
  • All
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein G Purified
Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot to detect tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 9 months at 2-8ºC from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. DO NOT FREEZE!
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
16-204 04053252366734

Documentation

Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate MSDS

Title

Safety Data Sheet (SDS) 

Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate Certificates of Analysis

TitleLot Number
Anti-Phosphotyrosine, recombinant 4G10., biotin conjugate - DAM1428201 DAM1428201
Anti-Phosphotyrosine, recombinant clone 4G10#174;, biotin conjugate - 1428201B 1428201B
Anti-Phosphotyrosine, recombinant clone 4G10#174;, biotin conjugate - 2155420 2155420
Anti-Phosphotyrosine, recombinant clone 4G10#174;, biotin conjugate - DAM1428201A DAM1428201A
Anti-Phosphotyrosine, recombinant clone 4G10reg;, biotin conjugate - 24362 24362
Anti-Phosphotyrosine, recombinant clone 4G10reg;, biotin conjugate - 28916 28916
Anti-Phosphotyrosine, recombinant clone 4G10reg;, biotin conjugate - 30082 30082
Anti-Phosphotyrosine, recombinant clone 4G10®, biotin conjugate - 2382606 2382606
Anti-Phosphotyrosine, recombinant clone 4G10®, biotin conjugate - 2275598 2275598
Anti-Phosphotyrosine, recombinant clone 4G10®, biotin conjugate Monoclonal Antibody 2884556

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Alternative Format

Catalogue Number Description
05-321 Anti-Phosphotyrosine Antibody, clone 4G10®
05-321MG Anti-Phosphotyrosine Antibody, clone 4G10®
05-321X Anti-Phosphotyrosine Antibody, clone 4G10®
05-777 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®
16-101 Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate
16-103 Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate
16-104 Anti-Phosphotyrosine Antibody, clone 4G10®, FITC conjugate
16-105 Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate
16-199 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate
16-205 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, FITC conjugate

Included Positive Control

Catalogue Number Description
12-302 EGF-Stimulated A431 Cell Lysate

Product Families

Phosphotyrosine Signaling Solutions

Study tyrosine phosphorylation using Millipore's specific and sensitive antibodies, including the popular clone 4G10®, and new 4G10 Platinum, available in various conjugations. See below for more.
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Phosphotyrosine Antibodies

Millipore’s phosphotyrosine antibodies, including the popular 4G10 clone, demonstrate sensitivity and detect multiple tyrosine phosphorylations on numerous substrates. See below for pTyr antibodies.
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies