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MABS1942-25UG Anti-Perilipin-1 Antibody, clone Peri 112.17.1

MABS1942-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS1942-25UG
DescriptionAnti-Perilipin-1 Antibody, clone Peri 112.17.1
Alternate Names
  • Lipid droplet-associated protein
Background InformationPerilipin-1 (UniProt: O60240; also known as Lipid droplet-associated protein) is encoded by the PLIN1 (also known as PERI, PLIN) gene (Gene ID: 5346) in human. Perilipins are lipid droplet (LD)- binding proteins that are required for optimal lipid storage and fatty acid release. In mammals the perilipin family of comprises five members: perilipin, adipophilin, TIP47, S3-12 and MLDP/OXPAT (also named PLIN1-5. Perilpin-1 is a modulator of adipocyte lipid metabolism that is detected in adipocytes and is also shown to be expressed in hepatocytes of human steatotic liver. It coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase. It is shown to play a role in unilocular lipid droplet formation by activating cell death inducing DFFA like effector c (CIDEC). Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. In adipocytes, perilipin-1 can undergo phosphorylation by protein kinase A that changes its conformation and permits lipolysis by hormone-sensitive lipase. Perilipin knockout mice are lean and display aberrant adipocyte lipolysis, enhanced leptin production, and resistance to diet-induced obesity. Mutations in PLIN1 gene are linked to familial lipodystrophy that is characterized by loss of subcutaneous adipose tissue primarily affecting the lower limbs, insulin-resistant diabetes mellitus, hypertriglyceridemia, and hypertension. (Ref.: Straub, BK et al. (2008). Hepatology. 47(6); 1936-1946; Tansey JT., et al. (2004). IUBMB Life. 56(7); 379-385).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Perilipin-1, clone Peri 112.17.1, Cat. No. MABS1942, is a mouse monoclonal antibody that detects Perilipin-1 and has been tested for use in Electron Microscopy, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Electron Microscopy
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Perilipin-1 in Immunoprecipitation applications (Heid, H., et. al. (2014). PLoS One. 9(2):e90386).

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Perilipin-1 in human breast and lipoma tissue sections.

Electron Microscopy Analysis: A representative lot detected Perilipin- positive lipid droplets by Immunoelectron Microscopy applications (Heid, H., et. al. (2014). PLoS One. 9(2):e90386).

Immunocytochemistry Analysis: A representative lot detected Perilipin-1 in Immunocytochemistry applications (Heid, H., et. al. (2014). PLoS One. 9(2):e90386).

Western Blotting Analysis: A representative lot detected Perilipin-1 in Western Blotting applications (Heid, H., et. al. (2014). PLoS One. 9(2):e90386).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to first 20 amino acids from the N-terminal region of human Perilipin-1.
ClonePeri 112.17.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone Peri 112.17.1 is a mouse monoclonal antibody that detects Perilipin-1. It targets an epitope within 20 amino acids from the N-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Bovine
  • Human
Species Reactivity NoteHuman, Bovine
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PLIN1
  • PERI
  • PLIN
Purification MethodProtein G purified
UniProt Number
Molecular Weight~ 65 KDa observed; 55.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH3T3/L1 cell lysate.

Western Blotting Analysis: A 1:500 Dilution of this antibody detected Perilipin-1 in NIH3T3/L1 cell lysate.


Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1942-25UG 04061841276715

Documentation

Anti-Perilipin-1 Antibody, clone Peri 112.17.1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Perilipin-1 Antibody, clone Peri 112.17.1 Certificates of Analysis

TitleLot Number
Anti-Perilipin-1, clone Peri 112.17.1 - Q3329774 Q3329774

References

Reference overviewPub Med ID
On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex.
Heid, H; Rickelt, S; Zimbelmann, R; Winter, S; Schumacher, H; Dörflinger, Y; Kuhn, C; Franke, WW
PLoS One  9  e90386  2014

Show Abstract
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