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07-1221 Anti-PP2A-B’ (B56) Antibody

Detect PP2A-B’ (B56) using this Anti-PP2A-B’ (B56) Antibody validated for use in WB.

Anti-PP2A-B’ (B56) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling, Kinases & Phosphatases Gene Symbol: MGC2184 , OTTHUMP00000022333, PP2A , PR53 , PTPA UniProt Number: Q15257
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07-1221
100 µg  
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Overview

Replacement Information
Replacement Information07-1221 is a recommended replacement for AB4088

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, H, M, RWBRbPurifiedPolyclonal Antibody
Description
Catalogue Number07-1221
ReplacesAB4088
DescriptionAnti-PP2A-B’ (B56) Antibody
Alternate Names
  • PP2A phosphatase activator
  • PP2A, subunit B
  • PP2A, subunit B', PR53 isoform
  • Phosphotyrosyl phosphatase activator
  • protein phosphatase 2A activator, regulatory subunit 4
  • protein phosphatase 2A, regulatory subunit B
  • protein phosphatase 2A, regulatory subunit B' (PR 53)
Background InformationPP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B'/PR61, and B''/PR72 . The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B' (or B56) family consists of at least seven splice variants derived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals. PTPA is a member of the B' family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.
References
Product Information
FormatPurified
Control
  • HeLa Cell Lysate
PresentationPurified rabbit polyclonal IgG in PBS with 0.08% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect PP2A-B’ (B56) using this Anti-PP2A-B’ (B56) Antibody validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenThe immunogen used for the purified peptide conjugated to KLH corresponds to the sequence NH2-Val-Ser-Ser-Pro-His-Phe-Gln-Val-Ala-Glu- Arg-Ala-Leu-Tyr-COOH.
EpitopeInternal
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody is specific to PP2A-B’ subunits.
IsotypeIgG
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily.
Gene Symbol
  • MGC2184
  • OTTHUMP00000022333
  • PP2A
  • PR53
  • PTPA
Purification MethodPurified
UniProt Number
UniProt SummaryFUNCTION: Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer in presence of ATP and Mg2+ (in vitro).
SUBUNIT STRUCTURE:Associates with PP2A heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A).
TISUE SPECIFICITY: Widely expressed.
SEQUENCE SIMILARITY: Belongs to the PTPA-type PPIase family.
Molecular Weight~56 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500-1:1,500 dilution of this antibody detected PP2A-B' (B56) in 10 μg of multiple cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-1221 04053252324192

Documentation

Anti-PP2A-B’ (B56) Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-PP2A-B’ (B56) Antibody Certificates of Analysis

TitleLot Number
Anti-PP2A-B#8217; (B56) - 2148310 2148310
Anti-PP2A-B#8217; (B56) - NG1590626 NG1590626
Anti-PP2A-B#8217; (B56) - NG1653545 NG1653545
Anti-PP2A-B#8217; (B56) - NG1698984 NG1698984
Anti-PP2A-B#8217; (B56) - NG1752512 NG1752512
Anti-PP2A-B#8217; (B56) - NG1882237 NG1882237
Anti-PP2A-B’ (B56) 3077398
Anti-PP2A-B’ (B56) - 3170226 3170226
Anti-PP2A-B’ (B56) - 3615440 3615440
Anti-PP2A-B’ (B56) -2608440 2608440
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