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ABT146 Anti-Occludin Antibody

This Anti-Occludin Antibody is validated for use in Western Blotting for the detection of Occludin.

Anti-Occludin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: OCLN UniProt Number: Q16625
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ABT146
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT146
DescriptionAnti-Occludin Antibody
Alternate Names
  • Occludin
Background InformationOccludin is a 65-kDa integral plasma-membrane protein located at the tight junctions. It is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Occludin is believed to prevent the transport of macromolecules through the tight junction. Encoded by the OLCN gene, occludin spans the plasma membrane four times, forming two extracellular loops and exposing its NH2 and COOH terminus to the cytosol. This cytoplasmic tail binds directly to the ZO family of proteins, which link the protein complex to the actin cytoskeleton. Phosphorylation/dephosphorylation plays a major role in regulation of occludin and tight junctions. Expression of occludin is required for infection of liver cells by hepatitis C virus (HCV).
References
Product Information
FormatAffinity Purified
Control
  • Human kidney tissue lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Occludin Antibody is validated for use in Western Blotting for the detection of Occludin.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Occludin.
EpitopeN-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the N-terminus of Occludin.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Porcine, Primate, Equine, Canine, Bovine, Mouse, and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • OCLN
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~63 kDa observed. The calculated molecular weight is 63 kDa, however Occludin has been shown ranging from ~55 to ~65 kDa in western blots (Florian, P., et al. (2003). Biochemical and Biophysical Research Communications. 304:5-10).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human kidney tissue lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected Occludin in 10 µg of human kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT146 04053252819520