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MABF937 Anti-Notch 3 Antibody, ECD, clone 2G8

Anti-Notch 3 Antibody, ECD, clone 2G8 is an antibody against Notch 3 for use in Immunohistochemistry (Paraffin), Immunofluorescence, Western Blotting.

Anti-Notch 3 Antibody, ECD, clone 2G8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Nervous System Gene Symbol: NOTCH3, CASIL, CADASIL, IMF2 UniProt Number: Q9UM47
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MABF937
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), IF, WBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF937
DescriptionAnti-Notch 3 Antibody, ECD, clone 2G8
Alternate Names
  • Neurogenic locus notch homolog protein 3
  • Notch 3
  • Notch homolog 3
  • Notch 3 ECD
Background InformationNeurogenic locus notch homolog protein 3 (UniProt Q9UM47; also known as Notch 3, Notch homolog 3) is encoded by the NOTCH3 (also known as CASIL, CADASIL, IMF2) gene (Gene ID 4854) in human. Notch 3 is a transmembrane receptor required for arterial differentiation and maturation of vascular smooth muscle cells in small arteries. Notch 3 is initially produced with a signal peptide (a.a. 1-39), the removal of which yields the 2282-amino acid Notch 3 (a.a. 40-2321), which undergoes a constitutive proteolytical cleavage, generating the extracellular fragment (Notch3-ECD; a.a. 40-1628) and the transmembrane/cytosolic (Notch3-TMIC; a.a. 1629-2321) fragment that remain associated at the cell surface to form a heterodimeric receptor. The ECD fragment contains all 34 EGF-like domains and the three Lin/Notch (LNR) repeats, while the extracellular portion of TMIC contains 5 ankyrin repeats. Mutations in the NOTCH3 gene have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Pathogenic mutations predominantly affect cysteine residues within EGF-like repeats of Notch3-ECD, leading to Notch3-ECD multimerization and accumulation in the tunica media of vessel walls.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Notch 3 Antibody, ECD, clone 2G8 is an antibody against Notch 3 for use in Immunohistochemistry (Paraffin), Immunofluorescence, Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
  • Western Blotting
Application NotesImmunohistochemistry Analysis: 20 µg/mL of this antibody from a representative lot detected Notch 3 extracellular domain (ECD) deposits in blood vessels of CADASIL patients brain tissue sections (Courtesy of Institute for Stroke and Dementia Research, Clinical center of the Ludwig-Maximilians University Munich, Germany).
Immunohistochemistry Analysis: A representative lot detected the aggregated Notch 3 extracellular domain (ECD) deposits in brain blood vessels of CADASIL patients by immunohistochemistry staining of paraformaldehyde-fixed, paraffin-embedded brain tissue sections (Kast, J., et al. (2014). Acta Neuropathol. Commun. 2:96).
Immunofluorescence Analysis: A representative lot detected the aggregated Notch 3 extracellular domain (ECD) deposits in brain blood vessels of CADASIL patients by fluorescent immunohistochemistry staining of paraformaldehyde-fixed frozen brain tissue sections. The Notch3-EDC immunoreactivity was seen co-localized with that of LTBP-1, but not fibrillin-1 (Kast, J., et al. (2014). Acta Neuropathol. Commun. 2:96).
Western Blotting Analysis: A representative lot detected an elevated accumulation of aggregated Notch 3 extracellular domain (ECD) in brain blood vessel extracts from CADASIL patients when comparing to vessel extracts from non-diseased brains (Kast, J., et al. (2014). Acta Neuropathol. Commun. 2:96).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a sequence from the EGF-like domains 33-34 region of human Notch 3 ECD.
EpitopeExtracellular EGF-like domains 33-34.
Clone2G8
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 2G8 reacts with unprocessed Notch 3 and the extracellular (Notch 3-ECD; a.a. 40-1628) fragment, but not the transmembrane/cytosolic fragment (Notch 3-TMIC, also called Notch 3 extracellular truncation; a.a. 1629-2321) or the Notch 3 intracellular fragment (a.a. 1662-2321).
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NOTCH3
  • CASIL
  • CADASIL
  • IMF2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight243.6 kDa (Pre-form), 239.5 kDa (mature, unprocessed), 167.13 kDa (Notch 3-ECD) calculated. ~250 kDa (Notch 3-ECD) reported (Kast, J., et al. (2014). Acta Neuropathol. Commun. 2:96).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIdentity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be IgG2aκ.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF937 04055977330311

Documentation

Anti-Notch 3 Antibody, ECD, clone 2G8 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Notch 3 Antibody, ECD, clone 2G8 Certificates of Analysis

TitleLot Number
Anti-Notch 3, ECD, clone 2G8 - 3234483 3234483
Anti-Notch 3, ECD, clone 2G8 - 3886044 3886044
Anti-Notch 3, ECD, clone 2G8 - 3933242 3933242
Anti-Notch 3, ECD, clone 2G8 - 4094282 4094282
Anti-Notch 3, ECD, clone 2G8 -Q2659003 Q2659003
Anti-Notch 3, ECD, clone 2G8 Monoclonal Antibody 3117566
Anti-Notch 3, ECD, clone 2G8 Monoclonal Antibody 3316666