Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AB5066P Anti-Norepinephrine Transporter Antibody

View Products on Sigmaaldrich.com
AB5066P
50 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RELISA, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5066P
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Norepinephrine Transporter Antibody
Alternate Names
  • NET
Product Information
FormatAffinity Purified
Control
  • Brain tissue
PresentationAffinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA .
Quality LevelMQ100
Applications
ApplicationThis Anti-Norepinephrine Transporter Antibody is validated for use in ELISA, WB for the detection of Norepinephrine Transporter.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern blot: 1-10 μg/mL (Chemiluminescence technique): NET can exist is at least two forms, a highly glycosylated ~80kDa form is the most common in brain, and a lessly glycosylated 54kDa form is also seen in some regions and tissues.



ELISA: 1:10,000-1:100,000 (50-100 ng immunogen peptide/well) Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenA 22 amino acid peptide sequence mapping to the 1st extracellular domain of rat NET.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificitySpecific for Norepinephrine Transporter (NET). Norepinephrine Transporter is a 617 amino acid long integral membrane protein belonging to the Sodium:Neurotransmitter Symporter family (Bruss et al. 1995; Melikian et al. 1994; Pacholczyk et al. 1991) The immunogen peptide shows no significant homology with other other known proteins.
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe SLC6A2 gene encodes a norepinephrine (noradrenaline) transporter, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis (Kim et al., 2006 [PubMed 17146058]).[supplied by OMIM]
Gene Symbol
  • SLC6A2
  • NAT1
  • NET
  • SLC6A5
  • NET1
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P23975 # Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals.
SIZE: 617 amino acids; 69332 Da
SUBUNIT: Interacts with PRKCABP.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DISEASE: SwissProt: P23975 # Defects in SLC6A2 are a cause of orthostatic intolerance (OI) [MIM:604715]. OI is a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. It is associated with postural tachycardia. Plasma norepinephrine concentration is abnormally high.
SIMILARITY: SwissProt: P23975 ## Belongs to the sodium:neurotransmitter symporter (SNF) family.
MISCELLANEOUS: This protein is the target of psychomotor stimulants such as amphetamines or cocaine.
Molecular Weight80 & 54 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size50 µg
Global Trade Item Number
Catalogue Number GTIN
AB5066P 04053252326257