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05-710 Anti-NG2 Antibody, clone 132.38

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05-710
200 µg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RIHC, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-710
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-NG2 Antibody, clone 132.38
References
Product Information
FormatPurified
Control
  • Brain tissue
PresentationProtein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative.
Quality LevelMQ100
Applications
ApplicationAnti-NG2 Antibody, clone 132.38 detects level of NG2 & has been published & validated for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesCross-reactivity expected with mouse.
Biological Information
ImmunogenHEK293 cells expressing a truncated integral membrane form of NG2 consisting of amino acids 1592-2222
Clone132.38
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes NG2.
IsotypeIgG1
Species Reactivity
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryA human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Gene Symbol
  • CSPG4
  • MCSP
  • HMW-MAA
  • MCSPG
  • MSK16
  • NG2
  • MEL-CSPG
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.


SIZE: 2322 amino acids; 250497 Da

SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.

SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).

TISSUE SPECIFICITY: Detected only in malignant melanoma cells.



PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity).


SIMILARITY: SwissProt: Q6UVK1 ## Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains.

MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells.
Molecular Weight~270-300 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on whole rat brain preparation
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-710 04053252736537

Documentation

Anti-NG2 Antibody, clone 132.38 MSDS

Title

Safety Data Sheet (SDS) 

Anti-NG2 Antibody, clone 132.38 Certificates of Analysis

TitleLot Number
Anti-NG2, clone 132.38 2475728
Anti-NG2, clone 132.38 (mouse monoclonal IgG1) - 2450197 2450197
Anti-NG2, clone 132.38 (mouse monoclonal IgG1) 3027174
Anti-NG2, clone 132.38 (mouse monoclonal IgG1) 2901516
Anti-NG2, clone 132.38 (mouse monoclonal IgG1) 2796319
Anti-NG2, clone 132.38 (mouse monoclonal IgG1) - 2250527 2250527
Anti-NG2, clone 132.38 (mouse monoclonal IgG1) - 3195955 3195955
Anti-NG2, clone 132.38 (mouse monoclonal IgG1) - 3678888 3678888
Anti-NG2, clone 132.38 - 0701049378 0701049378
Anti-NG2, clone 132.38 - 2020328 2020328

References

Reference overviewApplicationSpeciesPub Med ID
Collagen signaling enhances tumor progression after anti-VEGF therapy in a murine model of pancreatic ductal adenocarcinoma.
Aguilera, KY; Rivera, LB; Hur, H; Carbon, JG; Toombs, JE; Goldstein, CD; Dellinger, MT; Castrillon, DH; Brekken, RA
Cancer research  74  1032-44  2014

Show Abstract
24346431 24346431
Histone deacetylase expression in white matter oligodendrocytes after stroke.
Kassis, H; Chopp, M; Liu, XS; Shehadah, A; Roberts, C; Zhang, ZG
Neurochemistry international  77  17-23  2014

Show Abstract
24657831 24657831
The expression of spinal methyl-CpG-binding protein 2, DNA methyltransferases and histone deacetylases is modulated in persistent pain states.
Tochiki, KK; Cunningham, J; Hunt, SP; Géranton, SM
Molecular pain  8  14  2012

Show Abstract
22369085 22369085
Sensory and cortical activation of distinct glial cell subtypes in the somatosensory thalamus of young rats.
H Rheinallt Parri,Timothy M Gould,Vincenzo Crunelli
The European journal of neuroscience  32  2010

Show Abstract Full Text Article
20608967 20608967
In vivo intracellular recording suggests that gray matter astrocytes in mature cerebral cortex and hippocampus are electrophysiologically homogeneous.
Mishima, T; Hirase, H
The Journal of neuroscience : the official journal of the Society for Neuroscience  30  3093-100  2010

Show Abstract
Immunohistochemistry20181606 20181606
JAM-A is a novel surface marker for NG2-Glia in the adult mouse brain.
Stelzer, Sandra, et al.
BMC Neurosci, 11: 27 (2010)  2010

Show Abstract
20184779 20184779
A powerful transgenic tool for fate mapping and functional analysis of newly generated neurons.
Zhang, J; Giesert, F; Kloos, K; Vogt Weisenhorn, DM; Aigner, L; Wurst, W; Couillard-Despres, S
BMC neuroscience  11  158  2010

Show Abstract
Immunohistochemistry21194452 21194452
Genesis of neuronal and glial progenitors in the cerebellar cortex of peripuberal and adult rabbits.
Ponti, G; Peretto, P; Bonfanti, L
PloS one  3  e2366  2008

Show Abstract
Rabbit18523645 18523645
Multiple regions of the NG2 proteoglycan inhibit neurite growth and induce growth cone collapse
Ughrin, Yvonne M, et al
J Neurosci, 23:175-86 (2003)  2003

Immunohistochemistry (Tissue)Rat12514214 12514214
The oligodendrocyte precursor cell in health and disease
Levine, J M, et al
Trends Neurosci, 24:39-47 (2001)  2001

11163886 11163886

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Product Families

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies