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NE1019 Anti-Myelin Basic Protein Mouse mAb (SMI-99)

Overview

Key Spec Table

Species ReactivityHostAntibody Type
Gp, MaMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
NE1019-100ULCN 塑膠安瓿;塑膠針藥瓶 100 ul
Description
OverviewRecognizes the ~21 kDa myelin basic protein in rat brain tissue. Does not recognize the 14 kDa form. Recognizes most mammalian species.
Catalogue NumberNE1019
Brand Family Calbiochem®
Application Data
Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.
References
ReferencesEvers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197.
Shin, R.W., et al. 1991. Lab. Invest. 64, 693.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlRat brain
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (1:1000)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000)
Frozen Sections (1:1000)
Paraffin Sections (1:1000)
Application CommentsRecognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenhuman myelin basic protein
ImmunogenHuman
EpitopeWithin amino acids 131-136
CloneSMI-99
HostMouse
IsotypeIgG2b
Species Reactivity
  • Guinea Pig
  • Mammals
Antibody TypeMonoclonal Antibody
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
NE1019-100ULCN 04055977209884