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MAB3418-25UG Anti-MAP-2, clone AP20

Anti-MAP2 Antibody, clone AP20 detects level of MAP2 & has been published & validated for use in IH & WB.

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MAB3418-25UG
25 μg  
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Description
Catalogue NumberMAB3418-25UG
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-MAP-2, clone AP20
Alternate Names
  • Microtubule Associated Protein
  • MAP2A and 2B
Background InformationMAP-2 (microtubule associated protein-2) is one of several high molecular weight proteins that play an important role in brain neuron microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2. MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds. (Caceres, 1986; Dotti, 1987).
References
Product Information
FormatPurified
Control
  • Brain, Neuronal culture, Human glioblastoma T98G cells.
PresentationPurified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, pH 7.6, 0.25 M NaCl with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MAP2 Antibody, clone AP20 detects level of MAP2 & has been published & validated for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern Blot:
60 ng/mL. In SDS-PAGE, MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 280-300 kD representing the MAP-2A and -2B isoforms but not the 70 kDa MAP-2C isoform. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Users should run 4%-20% SDS-PAGE gradient gels.

Immunohistochemistry:
5 µg/mL (4% PFA fixed frozen sections).

Immunohistochemistry:
5 µg/mL of a previous lot was used. Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.

Optimal working concentrations must be determined by the end user.
Biological Information
ImmunogenBovine brain microtubule protein.
CloneAP20
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityMAB3418 binds specifically to MAP-2.
IsotypeIgG
Species Reactivity
  • Bovine
  • Chicken
  • Human
  • Mouse
  • Rat
  • Xenopus
  • Quail
Species Reactivity NoteRat and mouse. Expected to cross-react with human, cow, chicken, quail, and Xenopus.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
Gene Symbol
  • MAP2
  • MAP2B
  • MAP-2
  • MAP2C
  • DKFZp686I2148
  • MAP2A
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
SIZE: 1827 amino acids; 199539 Da
PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.
Molecular Weight230 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on Rat Brain lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected MAP2 on 10 μg of Rat Brain lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3418-25UG 04054839337468

Documentation

Anti-MAP-2, clone AP20 Certificates of Analysis

TitleLot Number
Anti-MAP-2, clone AP20 - 3173528 3173528
Anti-MAP-2, clone AP20 - 3221956 3221956
Anti-MAP-2, clone AP20 - 3321793 3321793
Anti-MAP-2, clone AP20 - 3474129 3474129
Anti-MAP-2, clone AP20 - 3760532 3760532
Anti-MAP-2, clone AP20 - 3787023 3787023
Anti-MAP-2, clone AP20 - 3809265 3809265
Anti-MAP-2, clone AP20 - 3841827 3841827
Anti-MAP-2, clone AP20 - 3849131 3849131
Anti-MAP-2, clone AP20 - 3872434 3872434
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