Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABT1460-25UL Anti-LAMC2 Antibody, clone P2H

Anti-LAMC2, clone P2H, Cat. No. MABT1460, is a mouse monoclonal antibody that detects Laminin subunit g2 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

View Products on Sigmaaldrich.com
MABT1460-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1460-25UL
DescriptionAnti-LAMC2 Antibody, clone P2H
Alternate Names
  • Laminin subunit gamma-2
  • Cell-scattering factor 140 kDa subunit
  • CSF 140 kDa subunit
  • Epiligrin subunit gamma
  • Kalinin subunit gamma
  • Kalinin/nicein/epiligrin 100 kDa subunit
  • Ladsin 140 kDa subunit
  • Laminin B2t chain
  • Laminin-5 subunit gamma
  • Large adhesive scatter factor 140 kDa subunit
  • Nicein subunit gamma
Background InformationLaminin subunit g2 (UniProt: Q13753; also known as Cell-scattering factor 140 kDa subunit, CSF 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma) is encoded by the LAMC2 (also known as LAMB2T, LAMNB2) gene (Gene ID: 3918) in human. Laminin is a major basement membrane glycoprotein consisting of α, β, γ chains that are bound to each other by disulfide bonds. Over 15 different isoforms have been reported in the laminin family that are expressed in a tissue-specific manner and play different role in each tissue. γ² a subunit of Laminin-5 is reported to be frequently over-expressed as a monomer or as the β³-γ² heterodimer in invasive cancers and is regarded as an invasion marker. It has 8 EGF-like domains. The cell adhesion and motility activities of Laminin-5 are reported to be regulated by the proteolytic cleavage of the short arm of Laminin γ² chain by BMP-1 or MT1-MMP. This cleavage releases a 45 kDa N-terminal proteolytic fragment (γ²pf). The short arm and γ²pf can be further cleaved by serine proteases and by matrix metalloproteinases resulting in the release of small N-terminal fragments. γ²pf‐like fragments have been reported to be present in conditioned media of cultured cancer cells and in sera from human cancer subjects. Clone P2H targets the second N-terminal EGF-like repeat (NE2) of domain V of laminin 2. (Ref.: Sato, H., et al. (2014). Cancer Sci. 105(2); 168-175; Miyazaki, K., et al. (2016). Cancer Sci. 107(12); 1909-1918).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-LAMC2, clone P2H, Cat. No. MABT1460, is a mouse monoclonal antibody that detects Laminin subunit g2 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunocytochemistry
  • ELISA
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected LAMC2 in lung squamous cell carcinoma cell lysates (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

Western Blotting Analysis: A representative lot detected LAMC2 in Western Blotting applications (Miyazaki, K., et. al. (2016). Cancer Sci. 107(12):1909-1918).

Immunohistochemistry (Paraffin) Analysis: 10 µg/mL from a representative lot detected LAMC2 in normal human skin, skin cancer, normal breast, and breast adenocarcinoma tissues (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

ELISA Analysis: 5 µg/mL from a representative lot detected LAMC2 in Lm-gamma2pf and Lm-gamma2 in Lm332 condition medium from Lm332-expressing HEK293 cells (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

Immunocytochemistry Analysis: A representative lot detected LAMC2 in Immunocytochemistry applications (Miyazaki, K., et. al. (2016). Cancer Sci. 107(12):1909-1918).

Immunohistochemistry Applications: A representative lot detected LAMC2 in Immunohistochemistry applications of lung squamous cell carcinomas and lung adenocarcinomas (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged 45-kDa N-terminal fragment consisting of g2pf, domain V.
EpitopeN-terminal
CloneP2H
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone P2H is a mouse monoclonal antibody that detects Laminin-5, g2 chain. It targets an epitope within the N-terminal, NE2 in domain V.
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LAMC2
  • LAMB2T
  • LAMNB2
Purification MethodProtein G purified
UniProt Number
Molecular Weight130.98 kDa calculated for Laminin-5, g2 chain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Laminin-5 subunit g2 (LAMC2) in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1460-25UL 04065265872669