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07-1487 Anti-JAK3 Antibody

Detect JAK3 using this Anti-JAK3 Antibody validated for use in WB & IP.

Anti-JAK3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Immunological Signaling Gene Symbol: JAK3, JAKL, LJAK, JAK3_HUMAN, L-JAK, JAK-3, EC 2.7.10.2 UniProt Number: P52333
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07-1487
100 µL  
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Overview

Replacement Information
Replacement Information07-1487 is a recommended replacement for AB3806.

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IPRbSerumPolyclonal Antibody
Description
Catalogue Number07-1487
ReplacesAB3806
DescriptionAnti-JAK3 Antibody
Alternate Names
  • Janus Kinase 3
  • Janus kinase 3 (a protein tyrosine kinase, leukocyte)
  • Leukocyte janus kinase
  • Tyrosine-protein kinase JAK3
Background InformationJAKs are non-receptor tyrosine kinases which couple to cytokine receptors, and are activated by cytokines. JAK kinases (JAK1, JAK2, JAK3, Tyk2) have a kinase domain and a second, pseudokinase domain which is not enzymatically active. The principal effectors of JAKs are the STATs, which are direct activators of transcription, but JAKs also signal to the Ras-MAP Kinase pathway, and through Tec, to the PI3 Kinase pathway. JAK3 is required for signaling through cytokine receptors which share a common γ chain. JAK2 is required for signaling through a different subset of cytokine receptors, while JAK1 is essential for signaling from the IL-2, IL-6 and interferon receptors. The stress-activated protein kinase 1 (SAPK) family is also referred to as the jun N-terminal kinase family in light of the substrate preference of these serine/threonine kinases. In addition to regulating many biological processes including proliferation and apoptosis, the JNK/SAPK family has been implicated in many neurodegenerative diseases including Alzheimer disease, Parkinson's disease and Amyotrophic lateral sclerosis.
References
Product Information
FormatSerum
Control
  • HeLa cell lysate
PresentationRabbit polyclonal IgG whole antiserum in buffer containing sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect JAK3 using this Anti-JAK3 Antibody validated for use in WB & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation: 5-10 uL of a previous lot of this antibody was used in immunoprecipitation.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenSynthetic peptide corresponding to amino acids 782-797 of mouse JAK3 (SDPTPGIPSPRDELC).
HostRabbit
SpecificityRecognizes JAK3 (Janus Kinase 3).
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryJAK3 encodes Janus kinase 3, a tyrosine kinase that belongs to the Janus family. JAK3 functions in signal transduction and interacts with members of the STAT (signal transduction and activators of transcription) family. JAK3 is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations that abrogate Janus kinase 3 function cause an autosomal SCID (severe combined immunodeficiency disease).
Gene Symbol
  • JAK3
  • JAKL
  • LJAK
  • JAK3_HUMAN
  • L-JAK
  • JAK-3
  • EC 2.7.10.2
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P52333 # Tyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K.
SIZE: 1124 amino acids; 125099 Da
SUBUNIT: Interacts with STAM2 and MYO18A (By similarity). Interacts with SHB.
SUBCELLULAR LOCATION: Intracytoplasmic membrane; Peripheral membrane protein (By similarity). Note=Wholly intracellular, possibly membrane associated (By similarity).
TISSUE SPECIFICITY: In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B- and M-forms are detected in cells both of hematopoietic and epithelial origins.
DOMAIN: SwissProt: P52333 Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
PTM: Tyrosine phosphorylated in response to IL-2 and IL-4.
DISEASE: SwissProt: P52333 # Defects in JAK3 are a cause of autosomal recessive T-cell negative/B-cell positive type of severe combined immunodeficiency (SCID) [MIM:600802]; a condition characterized by the absence of circulating mature T-lymphocytes and NK cells, normal to elevated numbers of nonfunctional B-lymphocytes, and marked hypoplasia of lymphoid tissues.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain. & Contains 1 SH2 domain
Molecular Weight125 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis:
1:500 dilution of this lot detected JAK3 on 10 μg of HeLa lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-1487 04053252468605

Documentation

Anti-JAK3 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-JAK3 Antibody Certificates of Analysis

TitleLot Number
Anti-JAK3 - 2388932 2388932
Anti-JAK3 - 2246816 2246816
Anti-JAK3 - 2298425 2298425
Anti-JAK3 - 3195970 3195970
Anti-JAK3 - 3934329 3934329
Anti-JAK3 - DAM1605018 DAM1605018
Anti-JAK3 - NG1548938 NG1548938
Anti-JAK3 - NG1646186 NG1646186
Anti-JAK3 - NG1690473 NG1690473
Anti-JAK3 - NG1720567 NG1720567
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Technical Info

Title
JAK/STAT Signaling Research Focus