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MAB1976-AF555 Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate

Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate is an antibody against Integrin αVβ3 for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: ITGAV, MSK8, VNRA UniProt Number: P06756
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MAB1976-AF555
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Av, Rb, B, Ca, Ch, H, Mk, PoICCMAlexaFluor®555Monoclonal Antibody
Description
Catalogue NumberMAB1976-AF555
DescriptionAnti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate
Alternate Names
  • Integrin alpha-V
  • Vitronectin receptor subunit alpha
  • CD51
  • Integrin alpha-V heavy chain
  • Integrin alpha-V light chain
  • Integrin αVβ3
Background InformationIntegrin alpha-V (CD51, Vitronectin receptor subunit alpha; UniProt P06756) and Integrin beta-3 (CD61, GPIIIa, Platelet membrane glycoprotein IIIa; UniProt P05106) are encoded by the ITGAV (MSK8, VNRA; Gene ID 3685) and ITGB3 (GP3A; Gene ID 3690) gene, respectively, in human. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and a beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue. Due to its involvement in angiogenesis, integrin alphaVbeta3 is among of the most studied integrin receptors.
References
Product Information
FormatAlexaFluor®555
PresentationPurified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate is an antibody against Integrin αVβ3 for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin αVβ3 in HeLa cells.
The unconjugated antibody (Cat. No. MAB1976) is shown to be suitable also for functional assay, flow cytometry, immunofluorescence, immunohistochemistry, and immunoprecipitation applications.
Biological Information
ImmunogenPurified adhesion receptor from the human melanoma cell line M21 (Cheresh, D.A., and Spiro R.C. (1987). J. Biol. Chem. 262(36):17703-11).
CloneLM609
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityReacts with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, D.A., and Spiro R.C. (1987). J Biol Chem. 262(36):17703-11; Cheresh, D.A. (1987). Proc. Natl. Acad. Sci. U.S.A. 84(18):6471-6475). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, P.C., et al. (1994). Science. 264(5158):569-571). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits.
IsotypeIgG1
Species Reactivity
  • Avian
  • Rabbit
  • Bovine
  • Canine
  • Chicken
  • Human
  • Monkey
  • Pig
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ITGAV
  • MSK8
  • VNRA
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~116 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HepG2 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Integrin αVβ3 in HepG2 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1976-AF555 04055977294729

Documentation

Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate MSDS

Title

Safety Data Sheet (SDS) 

Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate Certificates of Analysis

TitleLot Number
Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate 2907161
Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate 3792043
Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate - 3230943 3230943
Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate - 3722057 3722057
Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate - 3845817 3845817
Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate -Q2611359 Q2611359

Brochure

Title
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

Posters

Title
Post-Translational Modifications

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies