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MAB2250 Anti-Integrin β1 Antibody, clone 21C8

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MAB2250
100 µg  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, IP, FUNC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2250
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Integrin β1 Antibody, clone 21C8
Alternate Names
  • CD29
Product Information
FormatPurified
PresentationPurified immunoglobulin from Protein A Sepharose™ chromatography. Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Integrin β1 Antibody, clone 21C8 is an antibody against Integrin β1 for use in FC, IP, FUNC, IH.
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
  • Affects Function
  • Immunohistochemistry
Application NotesImmunoprecipitation

Immunohistochemistry on frozen sections

Flow cytometry

Stimulates adhesion of cells to extracellular matrix proteins (Wilkins et al., 1996)

Does not bind reduced beta1 integrin on Western blot

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenJurkat T-leukemic cell line
Clone21C8
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LAMB2
  • S-laminin
  • LAMS
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P55268 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
SIZE: 1798 amino acids; 195981 Da
SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-2 is a subunit of laminin-3 (S-laminin), laminin-4 (S- merosin), and laminin-7 (KS-laminin).
SUBCELLULAR LOCATION: Secreted, extracellular space. Note=S- laminin is concentrated in the synaptic cleft of the neuromuscular junction.
DOMAIN: SwissProt: P55268 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI and IV are globular.
DISEASE: SwissProt: P55268 # Defects in LAMB2 are the cause of Pierson syndrome [MIM:609049]; also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome. & Defects in LAMB2 are a cause of congenital nephrotic syndrome [MIM:609049]. Congenital nephrotic syndrome constitutes a heterogeneous group of conditions having in common the disruption of normal glomerular permselectivity. Congenital nephrotic syndrome due to LAMB2 mutations may be associated with ocular abnormalities.
SIMILARITY: Contains 13 laminin EGF-like domains. & Contains 1 laminin IV type B domain. & Contains 1 laminin N-terminal domain.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MAB2250 04053252725746