Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAB2079-AF555 Anti-Integrin β1 Antibody, activated, clone HUTS-4, Alexa Fluor® 555

This Anti-Integrin β1 Antibody, activated, clone HUTS-4, Alexa Fluor® 555 is validated for use in Immunocytochemistry for the detection of Integrin β1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: ITGB1, FNRB, MDF2, MSK12, VLA-BETA, VLAB UniProt Number: P05556
View Products on Sigmaaldrich.com
MAB2079-AF555
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RICCMAlexaFluor®555Monoclonal Antibody
Description
Catalogue NumberMAB2079-AF555
DescriptionAnti-Integrin β1 Antibody, activated, clone HUTS-4, Alexa Fluor® 555
Alternate Names
  • Integrin beta-1
  • CD29
  • Glycoprotein Iia
  • GPIIA
  • Integrin VLA-4 subunit beta
  • Very late activation protein, beta polypeptide
Background InformationIntegrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.
References
Product Information
FormatAlexaFluor®555
PresentationPurified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Integrin β1 Antibody, activated, clone HUTS-4, Alexa Fluor® 555 is validated for use in Immunocytochemistry for the detection of Integrin β1.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin β1 in A431 cells.
The unconjugated antibody (Cat. No. MAB2079Z) is shown to be suitable also for Western blotting, ELISA, functional assay, and immunohistochemistry.
Biological Information
ImmunogenGenerated against human β1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues.
EpitopeA.a. 355-425 region of the common β1 subunit of VLA integrins.
CloneHUTS-4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone HUTS-4 is specific for active conformation of human beta1 integrins. The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit nonconvalently associated with the common beta1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg2+ or Mn2+, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies such as clone HUTS-4 which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. Clone HUTS-4 recognizes epitopes in the 355-425 region of the common beta1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli.
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ITGB1
  • FNRB
  • MDF2
  • MSK12
  • VLA-BETA
  • VLAB
Purification MethodProtein G purified
UniProt Number
Molecular Weight~88 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Integrin β1 in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB2079-AF555 04055977355611

Documentation

Anti-Integrin β1 Antibody, activated, clone HUTS-4, Alexa Fluor® 555 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Integrin β1 Antibody, activated, clone HUTS-4, Alexa Fluor® 555 Certificates of Analysis

TitleLot Number
Anti-Integrin β1, activated, -Q2696983 Q2696983
Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor® 555 - 3397933 3397933
Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor® 555 - 3750008 3750008
Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor® 555 - 3948832 3948832
Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor® 555 - 4113030 4113030
Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor® 555 Monoclonal Antibody 3089567
Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor® 555 Monoclonal Antibody 2975209