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MABF2674-25UL Anti-IL-18 Antibody, clone 11-4.1

Anti-IL-18, clone 11 4.1, Cat. No. MABF2674, is a mouse monoclonal antibody that detects Interleukin-18 and is tested for use in Immunocytochemistry, Immunoprecipitation, Inhibition Assay, and Western Blotting.

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MABF2674-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABF2674-25UL
DescriptionAnti-IL-18 Antibody, clone 11-4.1
Alternate Names
  • Interleukin-18
  • Iboctadekin
  • Interferon gamma-inducing factor
  • IFN-gamma-inducing factor
  • Interleukin-1 gamma
  • IL-1 gamma
Background InformationInterleukin-18 (UniProt: Q14116; also known as IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma) is encoded by the IL18 (also known as IGIF, IL1F4) gene (Gene ID: 3606) in human. IL-18 is a pro-inflammatory cytokine that is shown to evoke both innate and acquired responses. It is synthesized with a propeptide (aa 1-36), which is subsequently cleaved off by caspases 1 and 4 to generate the active mature form. IL-18 is reported to be constitutively expressed in monocytes, macrophages, epithelial cells, osteoblasts, keratinocytes, and synovial fibroblasts. Its expression has also been reported in adrenal cortical cells. Mature IL-18 acts by binding to the IL-18 receptor a (IL-18a) to form a low affinity binary complex that recruits IL-18b to form a high affinity complex that leads to the activation of NF-kB and MAP kinase pathways. IL-18 can synergize with IL-12 to induce interferon  synthesis from T-helper 1 and natural killer cells. Clone 11-4.1 is shown to block IL-18 evolved interferon release in a dose-dependent manner (IC50 = 14.2 nM). (Ref.: Nariai, Y., et al. (2019). Arch. Biochem. Biophys. 663; 71-82).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in PBS without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-IL-18, clone 11 4.1, Cat. No. MABF2674, is a mouse monoclonal antibody that detects Interleukin-18 and is tested for use in Immunocytochemistry, Immunoprecipitation, Inhibition Assay, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
  • Inhibition
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated IL-18 in Immunoprecipitation applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).

Immunocytochemistry Analysis: A representative lot detected IL-18 in Immunocytochemistry applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).

Western Blotting Analysis: A representative lot detected IL-18 in Western Blotting applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected IL-18 in HeLa cells.

Inhibition Assay: A representative lot inhibited IL-18 involved interferon release from KG-1 cells.(Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged full-length active recombinant human Interleukin-18 (without the propeptide).
EpitopeN-terminal half
Clone11–4.1
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 11-4.1 is a mouse monoclonal antibody that detects human IL-18 protein. It targets an epitope within 6 amino acids from the N-terminal half.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • IL18
  • IGIF
  • IL1F4
Purification MethodProtein G purified
UniProt Number
Molecular Weight~19 kDa observed; 22.33 kDa calculated (for full-length IL-18). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IL-18 in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2674-25UL 04065265944793