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MABE164 Anti-ID4 Antibody, clone DML07

Use Anti-ID4 Antibody, clone DML07 (Mouse Monoclonal Antibody) validated in WB to detect ID4 also known as DNA-binding protein inhibitor ID-4, Class B basic helix-loop-helix protein 27, bHLHb27.

Anti-ID4 Antibody, clone DML07 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors, Developmental Signaling Gene Symbol: ID4 UniProt Number: P47928
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MABE164
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE164
DescriptionAnti-ID4 Antibody, clone DML07
Alternate Names
  • DNA-binding protein inhibitor ID-4
  • Class B basic helix-loop-helix protein 27
  • bHLHb27
  • Inhibitor of DNA binding 4
Background InformationID4 is an inhibitor of differentiation protein, which, along with ID1, ID2 and ID3, plays a key role in the regulation of lineage commitment, cell fate decisions and in the timing of differentiation during neurogenesis, lymphopoiesis and neovascularisation (angiogenesis). ID proteins, induced by the bone morphogenic protein-SMAD pathway, have also been shown to cooperate with LIF to ensure that embryonic stem cells opt for self-renewal.
References
Product Information
FormatPurified
Control
  • Human lung tissue lysate
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-ID4 Antibody, clone DML07 (Mouse Monoclonal Antibody) validated in WB to detect ID4 also known as DNA-binding protein inhibitor ID-4, Class B basic helix-loop-helix protein 27, bHLHb27.
Key Applications
  • Western Blotting
Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected ID4 on 10 µg of mouse lymph node tissue lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human ID4.
CloneDML07
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryTranscription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 [PubMed 7665172]).[supplied by OMIM].
Gene Symbol
  • ID4
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.

SUBUNIT STRUCTURE: Heterodimer with other HLH proteins.

SUBCELLULAR LOCATION: Nucleus.

SEQUENCE SIMILARITIES: Contains 1 basic helix-loop-helix (bHLH) domain.
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human lung tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected ID4 on 10 µg of human lung tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE164 04053252420429

Documentation

Anti-ID4 Antibody, clone DML07 MSDS

Title

Safety Data Sheet (SDS) 

Anti-ID4 Antibody, clone DML07 Certificates of Analysis

TitleLot Number
Anti-ID4, clone DML07 - 2193355 2193355
Anti-ID4, clone DML07 - 3202278 3202278
Anti-ID4, clone DML07 - 4158970 4158970
Anti-ID4, clone DML07 - NRG1904653 NRG1904653
Anti-ID4, clone DML07 Monoclonal Antibody 2926477
Anti-ID4, clone DML07 Monoclonal Antibody 3109184
Anti-ID4, clone DML07, Monoclonal Antibody 2840150

Brochure

Title
New Products: Volume 3, 2012