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04-1664 Sigma-AldrichAnti-Histidine Tag Antibody, clone RM146

This Anti-Histidine Tag Antibody, clone RM146 is validated for use in Western Blotting, Immunoprecipitation for the detection of Histidine Tag.

Anti-Histidine Tag Antibody, clone RM146 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
Technical Information

Research Category: Secondary & Control Antibodies Research Sub-Category: Secondary Antibodies Adsorbed for Dual Labeling

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
A Broad Range Of Species	WB, IP	Rb	Purified	Monoclonal Antibody

Description
Catalogue Number	04-1664
Description	Anti-Histidine Tag Antibody, clone RM146

References

Product Information
Format	Purified
Presentation	Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-Histidine Tag Antibody, clone RM146 is validated for use in Western Blotting, Immunoprecipitation for the detection of Histidine Tag.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Immunoprecipitation Analysis (IP): 0.5-2 μg/mL from a representative lot detected Histidine Tag in HEK293T whole lysate control and IP by clone RM146.

Biological Information
Immunogen	Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Clone	RM146
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Specificity	This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Isotype	IgG
Species Reactivity	A Broad Range Of Species
Antibody Type	Monoclonal Antibody
Purification Method	Protein A Purfied
Molecular Weight	~52 and ~85 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HEK293T cell lysate untransfected with His-Tag fusion protein X, transfected with His-Tag fusion protein X, E. coli lysate without His-Tag Protein Y, and E. coli lysate with His-Tag Protein Y. Western Blotting Analysis (WB): 0.2 μg/mL of this antibody detected Histidine Tag in HEK293T cell lysate transfected with His-Tag fusion protein X and E. coli lysate with His-Tag Protein Y.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting in HEK293T cell lysate untransfected with His-Tag fusion protein X, transfected with His-Tag fusion protein X, E. coli lysate without His-Tag Protein Y, and E. coli lysate with His-Tag Protein Y.

Western Blotting Analysis (WB): 0.2 μg/mL of this antibody detected Histidine Tag in HEK293T cell lysate transfected with His-Tag fusion protein X and E. coli lysate with His-Tag Protein Y.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
04-1664	04055977181333

Documentation

Anti-Histidine Tag Antibody, clone RM146 MSDS

Title
Safety Data Sheet (SDS)

Anti-Histidine Tag Antibody, clone RM146 Certificates of Analysis

Title	Lot Number
Anti-Histidine Tag, clone RM146 - VP1908022	VP1908022
Anti-Histidine Tag, clone RM146 - VP1911081	VP1911081
Anti-Histidine Tag, clone RM146 - VP2108263	VP2108263
Anti-Histidine Tag, clone RM146 - VP2112173	VP2112173
Anti-Histidine Tag, clone RM146 - VP2202182	VP2202182
Anti-Histidine Tag, clone RM146 - VP2205198	VP2205198
Anti-Histidine Tag, clone RM146 - VP2303021	VP2303021
Anti-Histidine Tag, clone RM146 - VP2309134	VP2309134
Anti-Histidine Tag, clone RM146 - VP2405022	VP2405022
Anti-Histidine Tag, clone RM146 -VP1511216	VP1511216

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.

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