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MABT12 Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4

Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4 detects level of Heparan Sulfate Proteoglycan (Perlecan) & has been published & validated for use in IH.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: HSPG2, SJA, perlecan, SJS, HSPG, PRCAN, Perlecan, SJS1, PLC UniProt Number: P98160
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MABT12
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIHC, ICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT12
DescriptionAnti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4
Alternate Names
  • heparan sulfate proteoglycan 2 (domain V region)
  • perlecan proteoglycan
  • basement membrane-specific heparan sulfate proteoglycan core protein
  • HSPG
  • PLC
Background InformationPerlecan is one of the extracellular matrix forms of heparan sulfate proteoglycans (HSPGs). It is a large multidomain and binds to and cross-links many extracellular matrix (ECM) components and cell-surface molecules. Perlecan is synthesized by vascular endothelial and smooth muscle cells and deposited in the extracellular matrix within all mammalian tissues. HSPGs differ in their protein cores that are thought to determine the location of HSPG in either the cell membrane (syndecan and glypican) or the extracellular matrix (perlecan and dystroglycan). HSPGs found in the brain are dystroglycan, N-syndecan glypican, and perlecan. The composition of HS isolated from brain differs significantly from that of other organs and is developmentally regulated. Thus, differences in protein cores, disaccharide composition, the extent and position of sulfation, and the number of GAG chains diversify chemical structure and functions of HSPGs. The postulated functions of HSPG include cell proliferation, differentiation, adhesion, migration, and morphogenesis.
References
Product Information
FormatPurified
Control
  • Fresh-frozen normal human tonsil tissue
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4 detects level of Heparan Sulfate Proteoglycan (Perlecan) & has been published & validated for use in IH.
Key Applications
  • Immunohistochemistry
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (courtesy of Whitelock, J. Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia)

Western Blot Analysis: A previous lot was used by an independent laboratory on a 3-8% Tris-acetate gel in which the band appears above the 460 kDa marker. (courtesy of Whitelock, J. Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia)
Biological Information
ImmunogenFull length recombinant protein corresponding to human Perlecan.
Clone5D7-2E4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and Transthyretin, etc. and plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and Tardive dyskinesia.
Gene Symbol
  • HSPG2
  • SJA
  • perlecan
  • SJS
  • HSPG
  • PRCAN
  • Perlecan
  • SJS1
  • PLC
Purification MethodProtein G
UniProt Number
UniProt SummaryFUNCTION: This protein is an integral component of basement membranes. It is responsible for the fixed negative electrostatic charge and is involved in the charge-selective ultrafiltration properties. It serves as an attachment substrate for cells.

SIZE: 4391 amino acids; 468825 Da

SUBUNIT: Purified perlecan has a strong tendency to aggregate in dimers or stellate structures. It interacts with other basement membrane components such as laminin, prolargin and collagen type IV. Interacts with COL13A1, FGFBP1 and VWA1.

SUBCELLULAR LOCATION: Secreted, extracellular space.

TISSUE SPECIFICITY: Found in the basement membranes.

PTM: N- and O-glycosylated; contains three heparan sulfate chains.

DISEASE: Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses. & Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.

SIMILARITY: Contains 4 EGF-like domains. & Contains 22 Ig-like C2-type (immunoglobulin-like) domains. & Contains 11 laminin EGF-like domains. & Contains 3 laminin G-like domains. & Contains 3 laminin IV type A domains. & Contains 4 LDL-receptor class A domains. & Contains 1 SEA domain.
Molecular Weight 469 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in fresh-frozen normal human tonsil tissue.

Immunohistochemistry Analysis: 1:300 dilution of the antibody detected Perlecan in fresh-frozen normal human tonsil tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT12 04053252413254

Documentation

Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4 Certificates of Analysis

TitleLot Number
Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 2148265 2148265
Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 2352421 2352421
Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 2277889 2277889
Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 3041057 3041057
Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 3232376 3232376
Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 3862688 3862688
Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 3949246 3949246
Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 4037461 4037461
Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - NRG1791891 NRG1791891
Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 -2505448 2505448
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