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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Recognizes chemically reduced amino acid-(4)-HNE adducts. Does not recognize non-reduced adducts or reduced modifications arising from malondialdehyde (MDA) and structurally-related alkenes such as trans-2-noneal.
Catalogue Number
393207
Brand Family
Calbiochem®
Synonyms
Anti-4-Hydroxy-2-noneal Michael Adducts, Reduced
References
References
Toyokuni, S., et al. 2000. Antioxid. Redox. Signal2, 681. Neely, M.D., et al. 1999. J. Neurochem.72, 2323. Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun.212, 1068. Uchida, K., et al. 1995. Arch. Biochem. Biophys.324, 241. Uchida, K., et al. 1994. Biochemistry33, 12487.
Does not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogen
HNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced
Host
Rabbit
Isotype
IgG
Species Reactivity
A Broad Range Of Species
Antibody Type
Polyclonal Antibody
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
≤ -70°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Anti-HNE-Michael Adducts, Reduced Rabbit pAb Certificates of Analysis
Title
Lot Number
393207
References
Reference overview
Toyokuni, S., et al. 2000. Antioxid. Redox. Signal2, 681. Neely, M.D., et al. 1999. J. Neurochem.72, 2323. Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun.212, 1068. Uchida, K., et al. 1995. Arch. Biochem. Biophys.324, 241. Uchida, K., et al. 1994. Biochemistry33, 12487.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
The CNS is enriched in polyunsaturated fatty acids and is therefore particularly vulnerable to lipid peroxidation. 4-Hydroxy-2-noneal (HNE) is one of the major lipid peroxidation products with cytotoxic and mutagenic activity. It reacts with protein residues such as histidine to generate stable Michael adducts. HNE has been shown to inhibit neurite outgrowth and disrupt microtubules and tubulin modification, suggesting it may play a role in Alzheimer's disease.
Host
Rabbit
Immunogen
HNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced
Isotype
IgG
Species
a broad range of species
Form
Liquid
Formulation
Serum diluted in 50% glycerol, pH 7.5.
Preservative
None
Comments
Does not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.
Storage
Avoid freeze/thaw
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Toyokuni, S., et al. 2000. Antioxid. Redox. Signal2, 681. Neely, M.D., et al. 1999. J. Neurochem.72, 2323. Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun.212, 1068. Uchida, K., et al. 1995. Arch. Biochem. Biophys.324, 241. Uchida, K., et al. 1994. Biochemistry33, 12487.
