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MAB342 Anti-Galactocerebroside Antibody, clone mGalC

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MAB342
50 µg  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ch, B, H, M, R, RbELISA, ICC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB342
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Galactocerebroside Antibody, clone mGalC
Alternate Names
  • Galactoceramide
  • Galactocerebroside
  • Galactosylceramide
  • GalC
Background InformationGalactocerebroside (GalC) is a major galactosphingolipid of myelin which plays a role in myelination. GalC is a very useful, specific marker for oligodendroglial lineage.
Product Information
FormatPurified
Control
  • Neonatal cortex
PresentationPurified mouse monoclonal in buffer containing 10 mM Potassium Phosphate, 150 mM NaCl, pH 7.4 containing 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Galactocerebroside Antibody, clone mGalC is an antibody against Galactocerebroside for use in ELISA, IC, & IHC.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunohistochemistry:
0.5-10 μg/mL of a previous lot was used on formalin fixed frozen sections of a prevous lot. Can not be used on paraffin embedded tissue sections since the antigen is denatured during embedding and paraffin removal.

Immunocytochemistry:
0.5-10 μg/mL of a previous lot was used on 4% paraformaldehyde, acetic acid or ethanol fixed cultured cells.

ELISA:
A previous lot was used on purified galactocerebrosides.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynaptic plasma membranes from bovine hippocampus. Ranscht, B. et al. PNAS 79(8):2709-2713 (1982)
ClonemGalC
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityGalactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically with oligodendrocytes and Schwann cells.
IsotypeIgG3
Species Reactivity
  • Chicken
  • Bovine
  • Human
  • Mouse
  • Rat
  • Rabbit
Antibody TypeMonoclonal Antibody
Purification MethodProtein A Purfied
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Packaging Information
Material Size50 µg
Global Trade Item Number
Catalogue Number GTIN
MAB342 04053252660382