Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAB374-AF488 Anti-GAPDH Antibody, clone 6C5, Alexa Fluor® 488 conjugate

This Anti-GAPDH Antibody, clone 6C5, Alexa Fluor® 488 conjugate is validated for use in Immunocytochemistry for the detection of GAPDH.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: GAPDH, GAPD UniProt Number: P46406
View Products on Sigmaaldrich.com
MAB374-AF488
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberMAB374-AF488
DescriptionAnti-GAPDH Antibody, clone 6C5, Alexa Fluor® 488 conjugate
Alternate Names
  • Glyceraldehyde-3-phosphate dehydrogenase
  • GAPDH
  • Peptidyl-cysteine S-nitrosylase GAPDH
  • GAPDH
Background InformationGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Its binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic role in metabolism, it is also involved in the initial stages of apoptosis or oxidative stress response where it is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH can also bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders, including the beta-amyloid precursor protein and the Huntingtin protein where reduced function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity.
References
Product Information
FormatAlexaFluor®488
PresentationPurified mouse conjugate antibody in buffer containing PBS 15 mg/ml BSA and 0.1% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-GAPDH Antibody, clone 6C5, Alexa Fluor® 488 conjugate is validated for use in Immunocytochemistry for the detection of GAPDH.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GAPDH in NIH/3T3 cells.
The unconjugated version of this antibody (Cat. No. MAB374) has been demonstrated to perform in WB, ELISA, ICC, IHC, and IP.
Biological Information
ImmunogenGlyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.
Clone6C5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityGlyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.
IsotypeIgG1
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman and Mouse. Predicted to react with Porcine, Canine, Cat, Rat, Rabbit, and Fish based on 100% sequence homology. It has been reported that this antibody does not react with GAPDH from E. coli.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GAPDH
  • GAPD
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~38 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GAPDH in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB374-AF488 04055977182064

Documentation

Anti-GAPDH Antibody, clone 6C5, Alexa Fluor® 488 conjugate MSDS

Title

Safety Data Sheet (SDS) 

Anti-GAPDH Antibody, clone 6C5, Alexa Fluor® 488 conjugate Certificates of Analysis

TitleLot Number
Anti-GAPDH, clone 6C5, Alexa Fluor® -2743351 2743351
Anti-GAPDH, clone 6C5, Alexa Fluor® 488 conjugate - 3284234 3284234
Anti-GAPDH, clone 6C5, Alexa Fluor® 488 conjugate - 3514189 3514189
Anti-GAPDH, clone 6C5, Alexa Fluor® 488 conjugate - 3852790 3852790
Anti-GAPDH, clone 6C5, Alexa Fluor® 488 conjugate - 3888781 3888781
Anti-GAPDH, clone 6C5, Alexa Fluor® 488 conjugate - 3988982 3988982
Anti-GAPDH, clone 6C5, Alexa Fluor® 488 conjugate - 4149120 4149120
Anti-GAPDH, clone 6C5, Alexa Fluor® 488 conjugate - 4175796 4175796
Anti-GAPDH, clone 6C5, Alexa Fluor® 488 conjugate -Q2586378 Q2586378
Anti-GAPDH, clone 6C5, Alexa Fluor®488 conjugate 2998323
Show more

Brochure

Title
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.

Posters

Title
Post-Translational Modifications