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MABC602 Anti-Ferritin Heavy Chain Antibody, clone 1-2.3.1.2

This Anti-Ferritin Heavy Chain Antibody, clone 1-2.3.1.2 is validated for use in Western Blotting and Immunohistochemistry for the detection of Ferritin Heavy Chain.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: FTH1, FTH, FTHL6 UniProt Number: P02794
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MABC602
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC602
DescriptionAnti-Ferritin Heavy Chain Antibody, clone 1-2.3.1.2
Alternate Names
  • Ferritin heavy chain
  • Ferritin H subunit
  • Cell proliferation-inducing gene 15 protein
  • Ferritin heavy chain, N-terminally processed
Background InformationFerritin is a widely expressed, very large, multi-subunit, intracellular protein that stores iron and releases it in a controlled fashion. The protein is produced by almost all living organisms in some form. In humans, it acts as a buffer against iron deficiency and iron overload. Ferritin protein is a multi-subunit (24 oligomers) protein complex consisting of both large (heavy) and small (light) chains. It is primarily and intracellular protein.The subunits of Ferritin in vertebrates have an apparent molecular weight of 19 kDa or 21 kDa respectively; their sequences are about 50% homologous. When Ferritin is unbound by iron the protein is called Apoferritin. Mutations associated with the Ferritin Heavy Chain protein contribute to a syndrome called Hemochromatosis 5 which is a disorder of iron metabolism where too much iron is retained within the tissues resulting in a variety of syndromes which unfortunately manifest themselves late in life.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Ferritin Heavy Chain Antibody, clone 1-2.3.1.2 is validated for use in Western Blotting and Immunohistochemistry for the detection of Ferritin Heavy Chain.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Ferritin Heavy Chain in human bone marrow and human tonsil tissue.
Immunohistochemistry Analysis: A representative lot detected Ferritin Heavy Chain in erythroid blasts of acute myeloid leukaemia M6 tissue, macrophages in the lymph node tissue, and tumor & lung-associated macrophages (Wang, W., et al. (2013). Histopahtology. 62:931–940).
Biological Information
ImmunogenRecombinant protein corresponding to human Ferritin Heavy Chain.
Clone1-2.3.1.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FTH1
  • FTH
  • FTHL6
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~21 kDa observed. Uncharacterized band may be observed at ~200 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in U251 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ferritin Heavy Chain in 10 µg of U251 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC602 04053252986505

Documentation

Anti-Ferritin Heavy Chain Antibody, clone 1-2.3.1.2 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Ferritin Heavy Chain Antibody, clone 1-2.3.1.2 Certificates of Analysis

TitleLot Number
Anti-Ferritin Heavy Chain, clone 1-2.3.1.2 - Q2447815 Q2447815
Anti-Ferritin Heavy Chain, clone 1-2.3.1.2 - 3734487 3734487
Anti-Ferritin Heavy Chain, clone 1-2.3.1.2 - 3988957 3988957
Anti-Ferritin Heavy Chain, clone 1-2.3.1.2 - 4059290 4059290
Anti-Ferritin Heavy Chain, clone 1-2.3.1.2 Monoclonal Antibody 3134102
Anti-Ferritin Heavy Chain, clone 1-2.3.1.2 Monoclonal Antibody 3003758

References

Reference overviewPub Med ID
Ferritin H is a novel marker of early erythroid precursors and macrophages.
Wang, Wei, et al.
Histopathology, 62: 931-40 (2013)  2013

Show Abstract 		23611361 23611361