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MABC128 Anti-FIP200 Antibody, clone 14E11.2

Anti-FIP200 Antibody, clone 14E11.2 is an antibody against FIP200 for use in Western Blotting, ICC.

Anti-FIP200 Antibody, clone 14E11.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: RB1CC1, KIAA0203, RBICC UniProt Number: Q86YR4
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MABC128
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC128
DescriptionAnti-FIP200 Antibody, clone 14E11.2
Alternate Names
  • RB1-inducible coiled-coil protein
  • 1FAK family kinase-interacting protein of 200 kDa
  • FIP200
Background InformationFocal adhesion kinase family interacting protein of 200 kDa (FIP200) is a novel protein inhibitor for focal adhesion kinase (FAK). FIP200 inhibits kinase activity and associated cellular functions, such as cell adhesion, spreading, and motility in fibroblasts by binding directly to FAK.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-FIP200 Antibody, clone 14E11.2 is an antibody against FIP200 for use in Western Blotting, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected FIP200 in serum starved and non-serum starved HeLa cell lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FIP200 in NIH/3T3, A431, and HeLa cells.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected FIP200 in untreated and chloroquinone-treated HUVEC cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the interaction domain of human FIP200.
EpitopeInteraction domain
Clone14E11.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody binds to the interaction domain of FIP200, which is needed for the formation of the ULK-FIP200 complex under starved, autophagy conditions.
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RB1CC1
  • KIAA0203
  • RBICC
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~200 kDa observed. An uncharacterized band may be observed at ~50 kDa in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected FIP200 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC128 04053252698811

Documentation

Anti-FIP200 Antibody, clone 14E11.2 MSDS

Title

Safety Data Sheet (SDS) 

Anti-FIP200 Antibody, clone 14E11.2 Certificates of Analysis

TitleLot Number
Anti-FIP200, clone 14E11.2 - 3507302 3507302
Anti-FIP200, clone 14E11.2 - 3739722 3739722
Anti-FIP200, clone 14E11.2 - 4010086 4010086
Anti-FIP200, clone 14E11.2 - 4208491 4208491
Anti-FIP200, clone 14E11.2 - Q2058408 Q2058408
Anti-FIP200, clone 14E11.2 -2490007 2490007
Anti-FIP200, clone 14E11.2 -2621116 2621116
Anti-FIP200, clone 14E11.2 -2734723 2734723
Anti-FIP200, clone 14E11.2 -2851237 2851237
Anti-FIP200, clone 14E11.2 Monoclonal Antibody 2896085
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