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AB3832 Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific

Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific detects level of Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) & has been published & validated for use in IH(P) & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: SLC9A3R1, +exchanger), NHERF1, +H, NHERF, Na, NHERF-1, EBP50 UniProt Number: O14745
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AB3832
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, Mk, RIH(P), WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB3832
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific
References
Product Information
FormatAffinity Purified
Control
  • Cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve
PresentationLiquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/ml BSA, with 50% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific detects level of Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) & has been published & validated for use in IH(P) & WB.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesWestern blotting 1:1,000 (for best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight). The antibody recognizes 75KDa (Moesin, and ~80kDa Ezrln & Radixin) bands.

Immunohistochemistry (paraffin): 1:50

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenKLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Thr567 of human ezrin
Epitopephospho-specific
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Thr567 phosphorylated ezrin, Thr564 phosphorylated radixin, and Thr558 phosphorylated moesin. Antibody does not cross-react with other related phospho proteins such as merlin or band 4.1.
Species Reactivity
  • Human
  • Mouse
  • Monkey
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryMembers of the ezrin (VIL2; MIM 123900)-radixin (RDX; MIM 179410)-moesin (MSN; MIM 309845) (ERM) protein family are highly concentrated in the apical aspect of polarized epithelial cells. These cells are studded with microvilli containing bundles of actin filaments, which must attach to the membrane to assemble and maintain the microvilli. The ERM proteins, together with merlin, the NF2 (MIM 607379) gene product, are thought to be linkers between integral membrane and cytoskeletal proteins, and they bind directly to actin in vitro. Actin cytoskeleton reorganization requires the activation of a sodium/hydrogen exchanger (SLC9A3; MIM 182307). SLC9A3R1 is an ERM-binding protein.[supplied by OMIM]
Gene Symbol
  • SLC9A3R1
  • +exchanger)
  • NHERF1
  • +H
  • NHERF
  • Na
  • NHERF-1
  • EBP50
Modifications
  • Phosphorylation
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O14745 # Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling.
SIZE: 358 amino acids; 38868 Da
SUBUNIT: Homodimer, and heterodimer with SLC9A3R2. Binds the N- termini of VIL2, RDX and MSN. Binds the C-termini of PDGFRA, PDGFRB, ADRB2, NOS2 and CFTR. Binds ARHGAP17, EPI64, GNB2L1, OPRK1, GNAQ, CTNNB1 and PLCB3. Binds PDZK1 (By similarity). Interacts with CLCN3. Binds the C-terminus of PAG1. In resting T- cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Forms a complex with CFTR and SLC4A7. Forms a complex with SLC4A7 and ATP6V1B1.
SUBCELLULAR LOCATION: Intracytoplasmic membrane; Peripheral membrane protein. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Note=Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
TISSUE SPECIFICITY: Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
PTM: Phosphorylated on serine residues.
SIMILARITY: SwissProt: O14745 ## Contains 2 PDZ (DHR) domains.
Molecular Weight75KDa Moesin, and ~80kDa Ezrin & Radixin
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C for 12 months, do not aliquot.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB3832 04053252279188

Documentation

Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific MSDS

Title

Safety Data Sheet (SDS) 

Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific Certificates of Analysis

TitleLot Number
RABBIT ANTI- PHOSPHO-EZRIN (THR567)/RADIXIN (THR564)/MOESIN (THR558) - 2210332 2210332
RABBIT ANTI- PHOSPHO-EZRIN (THR567)/RADIXIN (THR564)/MOESIN (THR558) - 2986619 2986619
RABBIT ANTI- PHOSPHO-EZRIN (THR567)/RADIXIN (THR564)/MOESIN (THR558) - 3045783 3045783
RABBIT ANTI- PHOSPHO-EZRIN (THR567)/RADIXIN (THR564)/MOESIN (THR558) - 3510868 3510868
RABBIT ANTI- PHOSPHO-EZRIN (THR567)/RADIXIN (THR564)/MOESIN (THR558) - 3612300 3612300
RABBIT ANTI- PHOSPHO-EZRIN (THR567)/RADIXIN (THR564)/MOESIN (THR558) - 3813497 3813497
RABBIT ANTI- PHOSPHO-EZRIN (THR567)/RADIXIN (THR564)/MOESIN (THR558) - 4013427 4013427
RABBIT ANTI- PHOSPHO-EZRIN (THR567)/RADIXIN (THR564)/MOESIN (THR558) POLYCLONAL ANTIBODY 3086139
RABBIT ANTI- PHOSPHO-EZRIN (THR567)/RADIXIN (THR564)/MOESIN (THR558) POLYCLONAL ANTIBODY - 2002161 2002161
RABBIT ANTI- PHOSPHO-EZRIN (THR567)/RADIXIN (THR564)/MOESIN (THR558) POLYCLONAL ANTIBODY - 2008617 2008617
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Data Sheet

Title
RABBIT ANTI- PHOSPHO-EZRIN (THR567)/RADIXIN (THR564)/MOESIN (THR558) POLYCLONAL ANTIBODY; AB3832