Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AB3284-200UL Anti-Endothelin Receptor B Antibody

Anti-Endothelin Receptor B Antibody detects level of Endothelin Receptor B & has been published & validated for use in IH & WB.

Anti-Endothelin Receptor B Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: CNS Control of Metabolism, Hormones & Receptors Gene Symbol: EDNRB, ET-B, ABCDS, HSCR2, ETRB, HSCR, ETB UniProt Number: P24530
View Products on Sigmaaldrich.com
AB3284-200UL
200 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RIHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB3284-200UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Endothelin Receptor B Antibody
Alternate Names
  • ET-B
References
Product Information
FormatAffinity Purified
Control
  • Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality LevelMQ100
Applications
ApplicationAnti-Endothelin Receptor B Antibody detects level of Endothelin Receptor B & has been published & validated for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPurified peptide corresponding to amino acids 298-314 of the rat Endothelin Receptor B (Accession P21451).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Endothelin Receptor B (ET-B). The immunogen sequence is specific for ET-B and is not present in any other known protein.

SPECIES REACTIVITIES: The immunogen sequence is identical in all known vertebrate proteins. Reactivity with other species has not been confirmed.
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Three transcript variants encoding two different isoforms have been found for this gene. While both isoforms bind ET1, they exhibit different responses upon binding, suggesting that they may be functionally distinct.
Gene Symbol
  • EDNRB
  • ET-B
  • ABCDS
  • HSCR2
  • ETRB
  • HSCR
  • ETB
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P24530 # Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
SIZE: 442 amino acids; 49644 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
PTM: Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
DISEASE: SwissProt: P24530 # Defects in EDNRB are a cause of Waardenburg syndrome type IV (WS4) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4 is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). & Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). It is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child. & Defects in EDNRB are the cause of ABCD syndrome [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine.
SIMILARITY: SwissProt: P24530 ## Belongs to the G-protein coupled receptor 1 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB3284-200UL 04053252512346

Documentation

Anti-Endothelin Receptor B Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Endothelin Receptor B Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-ENDOTHELIN RECEPTOR B AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2420354 2420354
RABBIT ANTI-ENDOTHELIN RECEPTOR B - 3269425 3269425
RABBIT ANTI-ENDOTHELIN RECEPTOR B AFFINITY PURIFIED POLYCLONAL ANTIBODY 2929215
RABBIT ANTI-ENDOTHELIN RECEPTOR B AFFINITY PURIFIED POLYCLONAL ANTIBODY - 1995583 1995583
RABBIT ANTI-ENDOTHELIN RECEPTOR B AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2281618 2281618

Data Sheet

Title
RABBIT ANTI-ENDOTHELIN RECEPTOR B AFFINITY PURIFIED POLYCLONAL ANTIBODY