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05-298 Anti-α-Dystroglycan Antibody, clone VIA4-1

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05-298
200 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ca, Gp, H, M, R, RbIHC, WBMCulture SupernatantMonoclonal Antibody
Description
Catalogue Number05-298
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-α-Dystroglycan Antibody, clone VIA4-1
OverviewNote: This product may be used for research purposes only. Diagnostic use of this product requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.
Background InformationDystroglycan is a component of the dystrophin glycoprotein complex that serves as an adhesion molecule and links the extracellular matrix to the intracellular actin cytoskeleton. It is synthesized with a signal peptide (aa 1-29) that is subsequently cleaved off to generate the mature protein that is further autolytically cleaved (between 653-654) to produce alpha-dystroglycan (aa 30-653) and beta-dystroglycan (aa 654-895). Alpha-dystroglycan is an extracellular peripheral glycoprotein that binds to several extracellular matrix and synaptic proteins such as laminin, agrin, neurexin, and pikachurin. It is reported that its O-mannosyl glycosylation is required for its ligand- binding functions. Beta-dystroglycan is a single-pass type 1 transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton and serves as a cell adhesion receptor in both muscle and non-muscle tissues. In the cell, beta-dystroglycan binds to dystrophin that is linked to actin cytoskeleton. Hence, alpha/beta dystroglycans act as a molecular axis connecting extracellular matrix with the cytoskeleton across the plasma membrane. Defects in O-mannosyl glycan have been linked to various congenital muscular dystrophies caused by aberrant alpha-dystroglycan glycosylation. (Ref.: Sudo, A., et al. (2018). Human Mol. Genetics. 27(7); 7 1174-1185; Kanagawa, M., et al. (2010). J. Biol. Chem. 285(41); 31208-31216).
References
Product Information
FormatCulture Supernatant
HS Code3002 15 90
Control
  • Mouse, rat and human skeletal muscle tissue extracts or DU 145 (human prostate carcinoma tumor) cell lysate
PresentationCulture supernatant containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect α-Dystroglycan using this Anti-α-Dystroglycan Antibody, clone VIA4-1. This antibody has been published and validated for use in IH and WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Biological Information
ImmunogenRabbit skeletal muscle membrane preparation
CloneVIA4-1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes α-dystroglycan/LARGE-glycan.
IsotypeIgG1
Species Reactivity
  • Canine
  • Guinea Pig
  • Human
  • Mouse
  • Rat
  • Rabbit
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryDystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence.
Gene Symbol
  • DAG1
  • A3a
  • DAG
  • beta-dystroglycan
  • alpha-dystroglycan
  • AGRNR
  • 156DAG
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q14118 # Forms part of the dystrophin-associated protein complex (DAPC) which may link the cytoskeleton to the extracellular matrix. Alpha-dystroglycan functions as a laminin receptor. Binds to several types of arenaviruses. Is a target for the entry of Mycobacterium leprae into peripheral nerve Schwann cells.
SIZE: 895 amino acids; 97581 Da
SUBUNIT: Interacts with SGCD (By similarity). Interacts with AGR2 and AGR3.
SUBCELLULAR LOCATION: Alpha-dystroglycan: Secreted, extracellular space. & Beta-dystroglycan: Cell membrane; Single- pass type I membrane protein.
TISSUE SPECIFICITY: Expressed in a variety of fetal and adult tissues.
Molecular Weight156 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated on rabbit skeletal muscle in a western blot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-298 08436037123894

Documentation

Anti-α-Dystroglycan Antibody, clone VIA4-1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-α-Dystroglycan Antibody, clone VIA4-1 Certificates of Analysis

TitleLot Number
Anti-#945;-Dystroglycan, clone VIA4-1 - 2150158 2150158
Anti-#945;-Dystroglycan, clone VIA4-1 - DAM1834934 DAM1834934
Anti--Dystroglycan, clone VIA4-1 - 1951974 1951974
Anti--Dystroglycan, clone VIA4-1 - 1993896 1993896
Anti--Dystroglycan, clone VIA4-1 - 2089509 2089509
Anti--Dystroglycan, clone VIA4-1 - DAM1408802 DAM1408802
Anti--Dystroglycan, clone VIA4-1 - DAM1632063 DAM1632063
Anti--Dystroglycan, clone VIA4-1 - DAM1764385 DAM1764385
Anti--Dystroglycan, clone VIA4-1 - DAM179797926 DAM179797926
Anti--Dystroglycan, clone VIA4-1 - JBC1863401 JBC1863401

References

Reference overviewApplicationSpeciesPub Med ID
Absence of glial α-dystrobrevin causes abnormalities of the blood-brain barrier and progressive brain edema.
Lien, CF; Mohanta, SK; Frontczak-Baniewicz, M; Swinny, JD; Zablocka, B; Górecki, DC
The Journal of biological chemistry  287  41374-85  2012

Show Abstract
23043099 23043099
Quiescence and activation of stem and precursor cell populations in the subependymal zone of the mammalian brain are associated with distinct cellular and extracellular matrix signals.
Kazanis, Ilias, et al.
J. Neurosci., 30: 9771-81 (2010)  2010

Show Abstract
20660259 20660259
Synapse formation and clustering of neuroligin-2 in the absence of GABAA receptors.
Patrizi, Annarita, et al.
Proc. Natl. Acad. Sci. U.S.A., 105: 13151-6 (2008)  2008

Show Abstract
ImmunofluorescenceMouse18723687 18723687
Worldwide distribution and broader clinical spectrum of muscle-eye-brain disease.
Taniguchi, Kiyomi, et al.
Hum. Mol. Genet., 12: 527-34 (2003)  2003

Show Abstract
Immunohistochemistry (Tissue)12588800 12588800
Mechanistic distinctions between agrin and laminin-1 induced aggregation of acetylcholine receptors.
Lee, LK; Kunkel, DD; Stollberg, J
BMC neuroscience  3  10  2002

Show Abstract
12182759 12182759
Dystroglycan is present in rat thyroid and rat thyroid cells and responds to thyrotropin.
Collins, B J, et al.
Endocrinology, 142: 3152-62 (2001)  2001

Show Abstract
Immunocytochemistry, Immunoblotting (Western)11416038 11416038
A stoichiometric complex of neurexins and dystroglycan in brain
Sugita, S., et al
J Cell Biol, 154:435-45 (2001)  2001

Immunocytochemistry, Immunoblotting (Western)11470830 11470830
Dystrophic phenotype induced in vitro by antibody blockade of muscle alpha-dystroglycan-laminin interaction
Brown, S. C., et al
J Cell Sci, 112 ( Pt 2):209-16 (1999)  1999

9858474 9858474
MEK kinase 1, a substrate for DEVD-directed caspases, is involved in genotoxin-induced apoptosis.
Widmann, C, et al.
Mol. Cell. Biol., 18: 2416-29 (1998)  1998

Show Abstract
Immunoblotting (Western)9528810 9528810
Membrane organization of the dystrophin-glycoprotein complex.
Ervasti, J M and Campbell, K P
Cell, 66: 1121-31 (1991)  1991

Show Abstract
1913804 1913804