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MABC580 Anti-Clathrin Heavy Chain Antibody, clone 6F10.1

Anti-Clathrin Heavy Chain Antibody, clone 6F10.1 is an antibody against Clathrin Heavy Chain for use in Western Blotting, Flow Cytometry, Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: CLTC, CLH17, CLTCL2, KIAA0034 UniProt Number: Q00610
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MABC580
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWB, FC, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC580
DescriptionAnti-Clathrin Heavy Chain Antibody, clone 6F10.1
Alternate Names
  • Clathrin heavy chain 1
  • Clathrin Heavy Chain
  • Clathrin heavy chain on chromosome 17
  • CLH-17
Background InformationClathrin heavy chain 1 (UniProt Q00610; also known as Clathrin heavy chain on chromosome 17, Clathrin heavy polypeptide (Hc), Clathrin heavy polypeptide-like 2, CLH-17) is encoded by the CLTC (also known as CHC, CLH17, CLTCL2, KIAA0034) gene in human (Entrez Gene ID 1213). Clathrin is the major protein component of the polyhedral coat of coated pits and vesicles. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of various macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Clathrin Heavy Chain Antibody, clone 6F10.1 is an antibody against Clathrin Heavy Chain for use in Western Blotting, Flow Cytometry, Immunocytochemistry.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • Immunocytochemistry
Application NotesFlow Cytometry Analysis: 1.5 µg of this antibody from a representative lot detected Clathrin Heavy Chain in 1X10E6 Jurkat cells.
Immunocytochemistry Analysis: 25.0 µg/mL from a representative lot detected Clathrin Heavy Chain in HeLa, A431, HUVEC, and NIH/3T3 cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human Clathrin Heavy Chain near the C-terminus.
Clone6F10.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Rat
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CLTC
  • CLH17
  • CLTCL2
  • KIAA0034
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~190 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Clathrin Heavy Chain in 10 µg of PC12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC580 04055977166620

Documentation

Anti-Clathrin Heavy Chain Antibody, clone 6F10.1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Clathrin Heavy Chain Antibody, clone 6F10.1 Certificates of Analysis

TitleLot Number
Anti-Clathrin Heavy Chain, -Q2569081 Q2569081
Anti-Clathrin Heavy Chain, clone 6F10.1 - 3426770 3426770
Anti-Clathrin Heavy Chain, clone 6F10.1 - 3736706 3736706
Anti-Clathrin Heavy Chain, clone 6F10.1 Monoclonal Antibody 3065503

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.