Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
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Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
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-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Detection of human calnexin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1), Vero (lane 2), Rat-2 cells (lane 3) and L-929 cells (lane 4). Primary antibody: Anti-Calnexin, C-Terminal (575-593) Rabbit pAb (Cat. No. 208880) (1:1000). Detection: chemiluminescence.
References
References
Hammond, C., et al. 1994. J. Cell Biol. 126, 41. Ou, W.-J., et al. 1993. Nature 364, 771. David, V., et al. 1993. J. Biol. Chem.268, 9585. Ahluwalia, N., et al. 1992. J. Biol. Chem.267, 10914. Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA89, 8452. Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA89, 4734. Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.
Anti-Calnexin, C-Terminal (575-593) Rabbit pAb Certificates of Analysis
Title
Lot Number
208880
References
Reference overview
Hammond, C., et al. 1994. J. Cell Biol. 126, 41. Ou, W.-J., et al. 1993. Nature 364, 771. David, V., et al. 1993. J. Biol. Chem.268, 9585. Ahluwalia, N., et al. 1992. J. Biol. Chem.267, 10914. Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA89, 8452. Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA89, 4734. Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
31-January-2008 JSW
Synonyms
Anti-IP90, Anti-p88
Application
Immunoblotting (1 µg/ml)
Application Data
Detection of human calnexin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1), Vero (lane 2), Rat-2 cells (lane 3) and L-929 cells (lane 4). Primary antibody: Anti-Calnexin, C-Terminal (575-593) Rabbit pAb (Cat. No. 208880) (1:1000). Detection: chemiluminescence.
Description
Protein A purified rabbit polyclonal antibody. Recognizes the ~90 kDa calnexin protein.
Background
Calnexin is an ER transmembrane protein that transiently associates with newly synthesized, incompletely folded monomeric glycoproteins. Calnexin associates with the ER membrane phosphoglycoprotein, signal sequence receptor SSRµ, and has been identified as the ER protein associated with major histocompatibility complex (MHC) class I heavy chain and with the T-cell receptor and membrane immunoglobulin before assembly of these multimeric complexes. Hence, calnexin may represent a molecular chaperone in the ER membrane that is selective for incompletely folded oligomeric membrane proteins newly translocated to the ER.
Host
Rabbit
Immunogen species
Canine
Immunogen
a synthetic peptide (AEEDEILNRSPRNRKPRRE) corresponding to amino acids 575-593 of canine calnexin, conjugated to KLH
Variables associated with assay conditions will dictate the optimal working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Hammond, C., et al. 1994. J. Cell Biol. 126, 41. Ou, W.-J., et al. 1993. Nature 364, 771. David, V., et al. 1993. J. Biol. Chem.268, 9585. Ahluwalia, N., et al. 1992. J. Biol. Chem.267, 10914. Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA89, 8452. Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA89, 4734. Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.
