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04-1506 Anti-CTCF Antibody, clone 1.2.1.5.4

Anti-CTCF Antibody, clone 1.2.1.5.4 is a Mouse Monoclonal Antibody for detection of CTCF also known as 11-zinc finger protein, CCCTC-binding factor & has been validated in WB, IP & IHC.

Anti-CTCF Antibody, clone 1.2.1.5.4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology, Transcription Factors Gene Symbol: CTCF UniProt Number: P49711
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04-1506
100 µg  
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Overview

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IP, IHC, ChIPMPurifiedMonoclonal Antibody
Description
Catalogue Number04-1506
ReplacesMABE547
DescriptionAnti-CTCF Antibody, clone 1.2.1.5.4
Alternate Names
  • 11 zinc finger transcriptional repressor
  • 11-zinc finger protein
  • CCCTC-binding factor
  • CCCTC-binding factor (zinc finger protein)
  • CTCFL paralog
  • transcriptional repressor CTCF
Background InformationCTCF, also known as CCCTC-binding factor, contains 11 zinc finger (ZF) binding domains that allow it to bind different DNA target sequences and proteins. This multifunctional protein can act as a transcriptional activator or repressor. As a transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mutations of CTCF in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms tumors, suggesting a role for CTCF may as a tumor suppressor.
References
Product Information
FormatPurified
Control
  • K562 cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CTCF Antibody, clone 1.2.1.5.4 is a Mouse Monoclonal Antibody for detection of CTCF also known as 11-zinc finger protein, CCCTC-binding factor & has been validated in WB, IP & IHC.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry
  • Chromatin Immunoprecipitation (ChIP)
Application NotesChromatin Immunoprecipitation Analysis:
A representative lot was used by an independent laboratory in ChIP. (Pugacheva, E., et al. (2005). Human Molecular Genetics. 14(7):953–965.)
Biological Information
ImmunogenHis-tagged recombinant protein corresponding to human CTCF.
EpitopeUnknown
Clone1.2.1.5.4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes CTCF.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. [provided by RefSeq].
Gene Symbol
  • CTCF
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation- dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.r/>SIZE: 727 amino acids; 82785 Da

SUBCELLULAR LOCATION: Nucleus.

TISSUE SPECIFICITY:Ubiquitous. Absent in primary spermatocytes.

SIMILARITY: Belongs to the CTCF zinc-finger protein family. & Contains 11 C2H2-type zinc fingers.
Molecular Weight~ 130 kDa
Note: Poly(ADP-ribosyl)ated isoform, C-terminal truncation, or sumoylation will cause variable migration of CTCF protein on SDS–PAGE, which may present CTCF with different molecular weight on Western Blot. Modifications are dependent on cell type and growing condition.
The following molecular weights have been reported in the literature: 180 kDa (CTCF-180), 130 kDa (CTCF-130), 82 kDa (CTCF-82), 70 kDa (CTCF-70). See Klenova,E.M.,et al, (1997) Nucleic Acids Res. 25, 466–473 and Torrano,V,et al, (2006) Journal of Cell Science 119, 1746-1759) for additional details.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected CTCF on 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-1506 04053252370755

Documentation

Anti-CTCF Antibody, clone 1.2.1.5.4 MSDS

Title

Safety Data Sheet (SDS) 

Anti-CTCF Antibody, clone 1.2.1.5.4 Certificates of Analysis

TitleLot Number
Anti-CTCF, clone 1.2.1.5.4 2485681
Anti-CTCF, clone 1.2.1.5.4 - 2278551 2278551
Anti-CTCF, clone 1.2.1.5.4 - 3595126 3595126
Anti-CTCF, clone 1.2.1.5.4 - 4042180 4042180
Anti-CTCF, clone 1.2.1.5.4 - NG1944109 NG1944109
Anti-CTCF, clone 1.2.1.5.4 - NRG1688321 NRG1688321
Anti-CTCF, clone 1.2.1.5.4 -2514480 2514480
Anti-CTCF, clone 1.2.1.5.4 -2770832 2770832
Anti-CTCF, clone 1.2.1.5.4 -2815182 2815182
Anti-CTCF, clone 1.2.1.5.4 Monoclonal Antibody 2937345
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