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05-1413 Anti-CD45RA Antibody, Clone MEM 56

Anti-CD45RA Antibody, Clone MEM 56 detects level of CD45RA & has been published & validated for use in FC, IF, IH(P), IP & WB.

Anti-CD45RA Antibody, Clone MEM 56 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Immunological Signaling Gene Symbol: PTPRC, L-CA, LCA, CD45, LY5, T200, Glycoprotein, B220, GP180, EC 3.1.3.48 UniProt Number: P08575
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05-1413
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RFC, IF, IH(P), IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1413
Replaces04-1102
DescriptionAnti-CD45RA Antibody, Clone MEM 56
Alternate Names
  • CD45 antigen
  • Leukocyte common antigen precursor
  • SCID due to PTPRC deficiency
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • Human homolog of severe combined immunodeficiency due to PTPRC deficiency
  • Leukocyte-common antigen
  • Protein tyrosine phosphatase, receptor type C
  • Protein tyrosine phosphatase, receptor type C polypeptide
Background InformationCD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.
References
Product Information
FormatPurified
Control
  • RAW264 Cell Lysate
PresentationPurified mouse monoclonal IgG2b in phosphate buffered saline containing 10 mM sodium azide and 1 mg/mL bovine serum albumin.
We recommend that each laboratory determine an optimum working titre for use in its particular application.
Quality LevelMQ100
Applications
ApplicationAnti-CD45RA Antibody, Clone MEM 56 detects level of CD45RA & has been published & validated for use in FC, IF, IH(P), IP & WB.
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunofluorescence: Identification of CD4+ suppressor/inducer cells, by indirect immunofluorescence staining.

Immunoprecipitation: A previous lot of this antibody detected 220 kDa molecular weight component of the leucocyte common antigen complex in immunoprecipitation.

Flow Cytometry: Purification or depletion of CD4+ suppressor/inducer cells by cell sorting or the panning techniques was performed on a previous lot.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Identification of CD45R+ lymphocytes in cryostat sections of frozen tissue and in formalin fixed wax embedded tissue.

Optimal Staining of CD45RA Monoclonal Antibody: Tonsil
Biological Information
CloneMEM 56
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes the 220 kDa molecular weight component of the leucocyte common antigen complex expressed on some CD4+ T lymphocytes, B lymphocytes and CD8+ lymphocytes. The CD45R+/CD4+ lymphocytes functionally exert suppressor/inducer activity in in vitro assay systems.
Antigen distribution:
B-cells (lg+) > 95%
T-cells (E+) 70 + 10%
Peripheral blood lymphocytes 68 + 8%
T-cells (CD4+) 50 + 10%
Monocytes 50 + 20%
Thymocytes < 5%
Granulocytes < 1%
IsotypeIgG2b
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteMouse. Expected to react with human and rat based on sequence homology. .
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Gene Symbol
  • PTPRC
  • L-CA
  • LCA
  • CD45
  • LY5
  • T200
  • Glycoprotein
  • B220
  • GP180
  • EC 3.1.3.48
Purification MethodPurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08575 # Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.
SIZE: 1304 amino acids; 147254 Da
SUBUNIT: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P08575 The first PTPase domain interacts with SKAP1.
PTM: Heavily N- and O-glycosylated.
DISEASE: SwissProt: P08575 # Defects in PTPRC are a cause of autosomal recessive severe combined immunodeficiency T-cell-negative/B-cell- positive/NK cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. SCID refers to a genetically and clinically group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms, including Candida albicans, Pneumocystis carinii, and cytomegalovirus, among many others. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T- cell development. & Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. & Contains 2 fibronectin type-III domains. & Contains 2 tyrosine-protein phosphatase domains.
Molecular Weight~220 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on RAW 264 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected CD45RA on 10 μg of RAW 264
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsFor use within 1 month of date of purchase store at 2-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-1413 04053252270802

Documentation

Anti-CD45RA Antibody, Clone MEM 56 MSDS

Title

Safety Data Sheet (SDS) 

Anti-CD45RA Antibody, Clone MEM 56 Certificates of Analysis

TitleLot Number
Anti-CD45RA, Clone MEM 56 - NG1576287 NG1576287
Anti-CD45RA, Clone MEM 56 - NG1631264 NG1631264
Anti-CD45RA, clone MEM 56 - 2192169 2192169
Anti-CD45RA, clone MEM 56 - 2227163 2227163
Anti-CD45RA, clone MEM 56 - 2326997 2326997
Anti-CD45RA, clone MEM 56 - 2495669 2495669
Anti-CD45RA, clone MEM 56 - 3587166 3587166
Anti-CD45RA, clone MEM 56 - 3770293 3770293
Anti-CD45RA, clone MEM 56 - 3862547 3862547
Anti-CD45RA, clone MEM 56 - NG1853758 NG1853758
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies