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MABC1189 Anti-Bin3 Antibody, clone 3A4

Anti-Bin3, clone 3A4, Cat. No. MABC1189, is a mouse monocloanl antibody that detects Bridging integrator 3 (Bin3) protein and has been tested for use in Western Blotting.

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MABC1189
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABC1189
DescriptionAnti-Bin3 Antibody, clone 3A4
Alternate Names
  • Bridging integrator 3
Background InformationBridging integrator 3 (UniProt: Q9NQY0; also known as Bin3) is encoded by the BIN3 gene (Gene ID: 55909) in human. Bin3 is a cytoskeletal protein that is ubiquitously expressed except in brain and is involved in cytokinesis and septation where it has a role in the localization of F-actin. It contain one BAR domain (aa 9-232), which forms coiled-coil structures. The BAR domain interacts with and facilitates tubulation of curved membranes and also binds to small GTPases and other cell regulatory proteins in the cytosol and nucleus. Deletion of Bin3 in mice is shown to cause cataracts associated with actin defects. Bin 3 I shown to suppress lymphoma during aging and restricts the efficiency of lung carcinogenesis. It is also shown to restrict the proliferation and motility of oncogenically transformed cells. (Ref.: Ramalingam, A., et al. (2008). Cancer Res. 68 (6): 1683-1690).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Bin3, clone 3A4, Cat. No. MABC1189, is a mouse monocloanl antibody that detects Bridging integrator 3 (Bin3) protein and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 4 µg/mL from a representative lot detected Bin3 in 10 µg of MCF7 and HEK293 cell lysate.

Western Blotting Analysis: A representative lot detected Bin3 in Western Blotting applications (Ramalingam, A., et. al. (2008). Cancer Res. 68(6):1683-90).
Biological Information
ImmunogenGST-tagged full length recombinant human Bridging integrator 3 (Bin3).
Clone3A4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3A4 detects Bridging integrator 3 (Bin3) in human and mouse cells.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BIN3
Purification MethodProtein G purified
UniProt Number
Molecular Weight~30 kDa observed; 29.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected Bin3 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1189 04054839253034

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