Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AB10546 Anti-Bcl-2-associated transcription factor 1 Antibody

This Anti-Bcl-2-associated transcription factor 1 Antibody is validated for use in WB, IP, IH(P) for the detection of Bcl-2-associated transcription factor 1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: BCL2 & Inhibition Gene Symbol: BCLAF1, BTF, Btf UniProt Number: Q9NYF8
View Products on Sigmaaldrich.com
AB10546
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, B, CaWB, IP, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB10546
DescriptionAnti-Bcl-2-associated transcription factor 1 Antibody
Alternate Names
  • BCL2-associated transcription factor 1
  • bcl-2-associated transcription factor 1
Background InformationBcl-2-associated transcription factor 1 (Btf) is a ubiquitously expressed death-promoting transcriptional repressor. It has been shown to interact with anti-apoptotic proteins related to Bcl-2, DNA, adenovirus E1B 19K, and EMD. Btf has also been shown to be involved in E1A transformation of p53 or E1B 19K, an interaction that implicates Btf as being involved in transcriptional regulation and in promoting apoptosis. Expression of Btf is absent in some cancers, which may suggest a potential role for the protein in tumor suppression.
References
Product Information
FormatAffinity Purified
Control
  • HeLa cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Bcl-2-associated transcription factor 1 Antibody is validated for use in WB, IP, IH(P) for the detection of Bcl-2-associated transcription factor 1.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blot (Snap i.d.) Analysis: 0.5 µg/mL from a previous lot detected Bcl-2-associated transcription factor 1 in 10 µg of HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Bcl-2-associated transcription factor 1 from HeLa cell lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected Bcl-2-associated transcription factor 1 in neuronal and glial cells in normal human brain tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Bcl-2-associated transcription factor 1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Bovine
  • Canine
Species Reactivity NoteDemonstrated to react with human and mouse.
Predicted to react with bovine and canine based on 100% sequence homology.
Other homologies: Rat (81% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Gene Symbol
  • BCLAF1
  • BTF
  • Btf
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Death-promoting transcriptional repressor.

SUBUNIT STRUCTURE: Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA.

SUBCELLULAR LOCATION: Cytoplasm. Nucleus.

TISSUE SPECIFICTY: Ubiquitous.

SEQUENCE CAUTION: The sequence AAH47687.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

The sequence AAH47887.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

The sequence AAH56894.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

The sequence AAH63846.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

The sequence BAA11481.2 differs from that shown. Reason: Erroneous initiation.
Molecular Weight~120 kDa observed.
This protein has 4 isoforms: Isoform 1 (106 kDa calculated), Isoform 2 (106 kda calculated), Isoform 3 (100 kDa calculated), Isoform 4 (86 kDa calculated).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Bcl-2-associated transcription factor 1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB10546 04053252642500

Documentation

Anti-Bcl-2-associated transcription factor 1 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Bcl-2-associated transcription factor 1 Antibody Certificates of Analysis

TitleLot Number
Anti-Bcl-2-associated Transcription Factor 1 - 2461559 2461559
Anti-Bcl-2-associated -2631506 2631506
Anti-Bcl-2-associated -2768167 2768167
Anti-Bcl-2-associated -2785815 2785815
Anti-Bcl-2-associated -2817240 2817240
Anti-Bcl-2-associated Transcription Factor 1 - 3385829 3385829
Anti-Bcl-2-associated Transcription Factor 1 - 3484993 3484993
Anti-Bcl-2-associated Transcription Factor 1 - 3934478 3934478
Anti-Bcl-2-associated Transcription Factor 1 - 4168140 4168140
Anti-Bcl-2-associated Transcription Factor 1 - NRG1811379 NRG1811379
Show more