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AB2219 Anti-Aquaporin 1 Antibody

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AB2219
100 µg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB2219
ReplacesAB3065
Trade Name
  • Chemicon
DescriptionAnti-Aquaporin 1 Antibody
Alternate Names
  • Aquaporin-1
  • AQP-1
  • Aquaporin-CHIP
  • Water channel protein for red blood cells and kidney proximal tubule
Background InformationAquaporin 1 is an integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Aquaporin 1 Antibody detects level of Aquaporin 1 & has been published & validated for use in ELISA, WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting: 1-10 μg/mL using Chemiluminescence technique. Immunoblots reveal two bands, a lower 28 kDa band corresponding to unglycosylated form and an upper high molecular 35-60 kDa glycosylated component (Denker et al. 1988; Smith & Agre 1991) We recommend the use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Aquaporin 1 in human kidney and mouse kidney tissue.

Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the topological domain of rat Aquaporin 1.
EpitopeTopological domain
Concentration1.0 mg/mL
HostRabbit
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteHuman, Rat, Mouse. Predicted to react with Primate, Bovine, Sheep based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • AQP1
  • Aquaporin-CHIP
  • MGC26324
  • AQP-CHIP
  • CO
  • Aquaporin-1
  • CHIP28
  • AQP-1
Purification MethodImmunoAffinity Purified
UniProt Number
Molecular Weight28 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Aquaporin 1 in 10 µg of human kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB2219 04053252274879

Documentation

Anti-Aquaporin 1 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Aquaporin 1 Antibody Certificates of Analysis

TitleLot Number
Anti-Aquaporin 1 2477951
Anti-Aquaporin 1 - 2151868 2151868
Anti-Aquaporin 1 - 2384006 2384006
Anti-Aquaporin 1 - 2443065 2443065
Anti-Aquaporin 1 - 1981641 1981641
Anti-Aquaporin 1 - 2011150 2011150
Anti-Aquaporin 1 - 2050258 2050258
Anti-Aquaporin 1 - 2280483 2280483
Anti-Aquaporin 1 - 2324379 2324379
Anti-Aquaporin 1 - 2890911 2890911

References

Reference overviewApplicationPub Med ID
Two nested developmental waves demarcate a compartment boundary in the mouse lung.
Alanis, DM; Chang, DR; Akiyama, H; Krasnow, MA; Chen, J
Nature communications  5  3923  2014

Show Abstract
24879355 24879355
Expression of E-cadherin in pig kidney.
Lee, SY; Han, SM; Kim, JE; Chung, KY; Han, KH
Journal of veterinary science  14  381-6  2013

Show Abstract
23820247 23820247
Activation of hepatocyte growth factor receptor, c-met, in renal tubules is required for renoprotection after acute kidney injury.
Zhou, D; Tan, RJ; Lin, L; Zhou, L; Liu, Y
Kidney international  84  509-20  2013

Show Abstract
23715119 23715119
Loss of Klotho contributes to kidney injury by derepression of Wnt/β-catenin signaling.
Zhou, L; Li, Y; Zhou, D; Tan, RJ; Liu, Y
Journal of the American Society of Nephrology : JASN  24  771-85  2013

Show Abstract
23559584 23559584
Lung epithelial branching program antagonizes alveolar differentiation.
Chang, DR; Martinez Alanis, D; Miller, RK; Ji, H; Akiyama, H; McCrea, PD; Chen, J
Proceedings of the National Academy of Sciences of the United States of America  110  18042-51  2013

Show Abstract
Immunohistochemistry24058167 24058167
A new AQP1 null allele identified in a Gypsy woman who developed an anti-CO3 during her first pregnancy.
Saison, C; Peyrard, T; Landre, C; Ballif, BA; Schlosser, KA; Dettori, I; Chicheportiche, C; Nemeth, P; Cartron, JP; Arnaud, L
Vox sanguinis  103  137-44  2012

Show Abstract
22348807 22348807
Dissociation of embryonic kidney followed by re-aggregation as a method for chimeric analysis.
Jamie A Davies,Mathieu Unbekandt,Jessica Ineson,Michael Lusis,Melissa H Little
Methods in molecular biology (Clifton, N.J.)  886  2012

Show Abstract
22639257 22639257
BMP4 sufficiency to induce choroid plexus epithelial fate from embryonic stem cell-derived neuroepithelial progenitors.
Watanabe, M; Kang, YJ; Davies, LM; Meghpara, S; Lau, K; Chung, CY; Kathiriya, J; Hadjantonakis, AK; Monuki, ES
The Journal of neuroscience : the official journal of the Society for Neuroscience  32  15934-45  2012

Show Abstract
Immunofluorescence23136431 23136431
Tubule-specific ablation of endogenous β-catenin aggravates acute kidney injury in mice.
Zhou, D; Li, Y; Lin, L; Zhou, L; Igarashi, P; Liu, Y
Kidney international  82  537-47  2012

Show Abstract
Immunohistochemistry22622501 22622501
Fluid-percussion brain injury induces changes in aquaporin channel expression.
Oliva AA Jr, Kang Y, Truettner JS, Sanchez-Molano J, Furones C, Yool AJ, Atkins CM
Neuroscience  2011

Show Abstract
21329742 21329742