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16-318 Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate

Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate validated for use in IP.

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Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling
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16-318
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RIPMPurifiedMonoclonal Antibody
Description
Catalogue Number16-318
DescriptionAnti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate
Background InformationAkt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.
References
Product Information
FormatPurified
Control
  • NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
Presentation20% slurry; 1X PBS, 0.05% sodium azide.
Sufficient for 10 IPs.
Quality LevelMQ100
Applications
ApplicationDetect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate validated for use in IP.
Key Applications
  • Immunoprecipitation
Biological Information
ImmunogenGST-fusion protein corresponding to residues 1-149 of human Akt1
Cloneclone SKB1
HostMouse
SpecificityRecognizes Akt1/PKBα.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Chromatography
Molecular Weight~ 60 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by immunoprecipitation on 250 μg of NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
16-318 04053252518980