Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABE144 Anti-Ago3 Antibody, clone 4B1-F6

Anti-Ago3 Antibody, clone 4B1-F6 is a Mouse Monoclonal Antibody for detection of Ago3 also known as eIF-2C 3, eIF2C 3, FLJ12765, MGC86946 & has been validated in ICC & IP.

Anti-Ago3 Antibody, clone 4B1-F6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: EIF2C3, AGO3 UniProt Number: Q9H9G7
View Products on Sigmaaldrich.com
MABE144
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE144
DescriptionAnti-Ago3 Antibody, clone 4B1-F6
Alternate Names
  • eIF-2C 3
  • eIF2C 3
  • FLJ12765
  • MGC86946
Background InformationEukaryotic translation initiation factor 2C 3, Ago3, belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain.
Ago3 is indicated to play a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. Unlike Ago1 and Ago2, Ago3 and AG04 do not possess the ability to dissociate miRNA duplexes in a minimal in vitro system. Re-introduction of any Ago variant into embyronic (ES) cells deficient for expression of all four Ago variants will rescue miRNA silencing defects and reduce apoptosis.
References
Product Information
FormatPurified
Control
  • HeLa cells
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Ago3 Antibody, clone 4B1-F6 is a Mouse Monoclonal Antibody for detection of Ago3 also known as eIF-2C 3, eIF2C 3, FLJ12765, MGC86946 & has been validated in ICC & IP.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Azuma-Mukai, A., et al. (2008). Proc Natl Acad Sci. USA. 105(23):7964-7969.)
Biological Information
ImmunogenRecombinant protein corresponding to human Ago3.
Clone4B1-F6
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
Specificity This antibody has been shown to not cross react with Ago1, Ago2, or Ago4 (Azuma-Mukai A et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene.
Gene Symbol
  • EIF2C3
  • AGO3
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs.

SUBUNIT STRUCTURE: Interacts with EIF4B, IMP8, PRMT5 and TNRC6B.

SUBCELLULAR LOCATION: Cytoplasm > P-body.
Molecular Weight96 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Ago3 in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE144 04053252754395

Documentation

Anti-Ago3 Antibody, clone 4B1-F6 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Ago3 Antibody, clone 4B1-F6 Certificates of Analysis

TitleLot Number
Anti-Ago3, clone 4B1-F6 - 2391171 2391171
Anti-Ago3, clone 4B1-F6 - 2006946 2006946
Anti-Ago3, clone 4B1-F6 - 3310131 3310131
Anti-Ago3, clone 4B1-F6 - 3433738 3433738
Anti-Ago3, clone 4B1-F6 - 3808724 3808724
Anti-Ago3, clone 4B1-F6 - NRG1911092 NRG1911092
Anti-Ago3, clone 4B1-F6 -2524850 2524850
Anti-Ago3, clone 4B1-F6 -2775321 2775321
Anti-Ago3, clone 4B1-F6 -2786916 2786916
Anti-Ago3, clone 4B1-F6 Monoclonal Antibody 2929151

Brochure

Title
New Products: Volume 3, 2012