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MABT84 Anti-Aggrecan Antibody, clone 1-C-6

This Anti-Aggrecan Antibody, clone 1-C-6 is validated for use in IH, WB for the detection of Aggrecan.

Anti-Aggrecan Antibody, clone 1-C-6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: Acan, Agc, Agc1 UniProt Number: P07897
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MABT84
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, M, BIHC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT84
DescriptionAnti-Aggrecan Antibody, clone 1-C-6
Alternate Names
  • Aggrecan core protein
  • Cartilage-specific proteoglycan core protein
  • CSPCP
Background InformationAggregating chondroitin sulfate proteoglycan or aggrecan, is one of the most studied proteoglycans. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight.
References
Product Information
FormatPurified
Control
  • Normal mouse cartilage and normal rat cartilage tissues
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Aggrecan Antibody, clone 1-C-6 is validated for use in IH, WB for the detection of Aggrecan.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected Aggrecan on 10 µg of A1D4 protein fraction from Bovine tissue.
Biological Information
ImmunogenReduced and alkylated Aggrecan isolated from Swarm Rat Chondrosarcoma
Clone1-C-6
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Rat
  • Mouse
  • Bovine
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryAn extracellular matrix proteoglycan; cleaved by ADAMTS1 [RGD, Feb 2006].
Gene Symbol
  • Acan
  • Agc
  • Agc1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.

SUBUNIT STRUCTURE: Interacts with FBLN1 and COMP (By similarity).

SUBCELLULAR LOCATION: Secreted › extracellular space › extracellular matrix (By similarity).

DOMAIN: Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.

PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides.

SEQUENCE SIMILARITIES: Belongs to the aggrecan/versican proteoglycan family.

Contains 1 C-type lectin domain.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Contains 4 Link domains.

Contains 1 Sushi (CCP/SCR) domain.
Molecular WeightVarious
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in normal mouse cartilage and normal rat cartilage tissues.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Aggrecan in normal mouse cartilage and normal rat cartilage tissues.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT84 04053252633713

Documentation

Anti-Aggrecan Antibody, clone 1-C-6 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Aggrecan Antibody, clone 1-C-6 Certificates of Analysis

TitleLot Number
Anti-Aggrecan, clone 1-C-6 - 2246809 2246809
Anti-Aggrecan, clone 1-C-6 - 3184796 3184796
Anti-Aggrecan, clone 1-C-6 - 3200631 3200631
Anti-Aggrecan, clone 1-C-6 - 3695366 3695366
Anti-Aggrecan, clone 1-C-6 - 3756228 3756228
Anti-Aggrecan, clone 1-C-6 - 4061129 4061129
Anti-Aggrecan, clone 1-C-6 - NRG1935584 NRG1935584
Anti-Aggrecan, clone 1-C-6 -2545769 2545769
Anti-Aggrecan, clone 1-C-6 -2567126 2567126
Anti-Aggrecan, clone 1-C-6 -2605490 2605490
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