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MABE1057 Anti-ADA3 Antibody, clone 5C9/C8

Anti-ADA3, clone 5C9/C8, Cat. No. MABE1057, is a highly specific mouse monoclonal antibody that targets TADA3 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-ADA3 Antibody, clone 5C9/C8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: TADA3, ADA3, TADA3L UniProt Number: O75528
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MABE1057
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IH(P), ChIP, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1057
DescriptionAnti-ADA3 Antibody, clone 5C9/C8
Alternate Names
  • Transcriptional adapter 3
  • ADA3 homolog
  • ADA3-like protein
  • Alteration/deficiency in activation 3
  • hADA3
  • STAF54
  • Transcriptional adapter 3-like
Background InformationTranscriptional adapter 3 (UniProt O75528; also known as ADA3 homolog, ADA3-like protein, Alteration/deficiency in activation 3, hADA3, STAF54, Transcriptional adapter 3-like) is encoded by the TADA3 (also known as ADA3, TADA3L) gene (Gene ID 10474) in human. Alteration/deficiency in activation 3 (ADA3) is a component of several transcriptional co-activator and histone acetyltransferase (HAT) complexes and plays a critical role in in cell cycle regulation. Ada3 deletion in mice is embryonic lethal, and Ada3-deficient mouse embryonic fibroblasts (MEFs) exhiit a severe proliferation defect, dramatic changes in global histone acetylation, mitotic defects, as well as a delay in G2/M-to-G1 and G1-to-S transition. ADA3 also plays a role in genomic stability by controlling DNA repair checkpoints. ChIP-seq analsis reveals that ADA3 is significantly associated with human centromere regions across most chromosomes. In addition, ADA3 is found associated with CENP-B throughout all phases of the cell cycle, and CENP-B centromere binding decreased upon ADA3 knockdown. Wild-type human ADA3, but not CENP-B-binding deficient ADA3 mutant, prevented cell proliferation defects in MEFs following endogenous mouse ADA3 knockown. ADA3 overexpression and mislocalization correlates with poor prognosis in breast cancer patients.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-ADA3, clone 5C9/C8, Cat. No. MABE1057, is a highly specific mouse monoclonal antibody that targets TADA3 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry
Application NotesImmunohistochemistry Analysis: A representative lot detected nuclear ADA3 immunoreactivity in formalin-fixed, paraffin-embedded human breast carcinoma tissue section (Courtesy of Vimla Band, Ph.D., University of Nebraska USA).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected ADA3 occupancy at the X chromosome centromere HOR region, the kinetochore assembly site also occupied by CENP-A and CENP-B, using TERT-immortalized human mammary epithelial cell 76N-TERT chromatin preparation. shRNA-mediated ADA3 knockdown led to a reduction in CENP-B, but not CENP-A, recruitment to the HOR region (Mohibi, S., et al. (2015). J. Biol. Chem. 290(47):28299-28310).

Immunocytochemistry Analysis: A representative lot detected ADA3 nuclear immunoreactivity in untransfected, but not ADA3 shRNA-transfected, TERT-immortalized human mammary epithelial 76N-TERT cells by fluorescent immunocytochemistry staining of 4% formaldehyde-fixed cells. Dual fluorescent staining revealed ADA3 and CENP-B nuclear colocalization (Mohibi, S., et al. (2015). J. Biol. Chem. 290(47):28299-28310).

Western Blotting Analysis: Representative lots detected both the endogenous mouse ADA3 (mADA3) and the exogenously expressed human ADA3 (hADA3) in Ada3FL/FL MEFs virally infected to express FLAG-tagged full-length or a.a. 111-432 hADA3 constructs. Cre recombinase expression downregulated only mADA3, but not hADA3 (Mohibi, S., et al. (2015). J. Biol. Chem. 290(47):28299-28310; Mohibi, S., et al. (2012). J. Biol. Chem. 287(35):29442-29456).

Western Blotting Analysis: A representative lot detected ADA3 expression levels among a panel of mouse tissues (Mohibi, S., et al. (2012). J. Biol. Chem. 287(35):29442-29456).
Biological Information
ImmunogenFull-length recombinant human ADA3.
Clone5C9/C8
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5C9/C8 specifically detected Cre recombinase expression-induced ADA3 downregulation in Ada3FL/FL MEFs. Clone 5C9/C8 immunostained the nuclei of untransfected, but not ADA3 shRNA-transfected, 76N-TERT human mammary epithelial cells (Mohibi, S., et al. (2015). J. Biol. Chem. 290(47):28299-28310; Mohibi, S., et al. (2012). J. Biol. Chem. 287(35):29442-29456).
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TADA3
  • ADA3
  • TADA3L
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~55 kDa observed. 48.90 kDa (human and mouse isoform 1) calculated. The larger-than-calculated band size is consistent with that reported in the literature (Mohibi, S., et al. (2015). J. Biol. Chem. 290(47):28299-28310). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ADA3 in 10 µg of MCF-7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1057 04054839069147