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CB1024 Anti-ACTN4 Mouse mAb (4D10)

Anti-ACTN4, mouse monoclonal, clone 4D10, recognizes the ~90-100 kDa ACTN4 in PC-12, HeLa, Raw 264.7, MCF-7, NIH3T3 cells & in human testes. It is validated for ELISA, WB, ICC, and paraffin sections.

Anti-ACTN4 Mouse mAb (4D10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Actinin α4

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CB1024
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
CB1024-100UGCN 100 μg
Description
OverviewRecognizes the ~90-100 kDa ACTN4 protein in PC-12, HeLa, Raw 264.7, MCF-7, and NIH3T3 cells and human testes tissue.
Catalogue NumberCB1024
Brand Family Calbiochem®
SynonymsAnti-Actinin α4
Application Data
Detection of rat ACTN4 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse ACTN4 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Priamry antibody: Anti-ACTN4 Mouse mAb (AD10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human ACTN4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (3 µg/ml). Detection: colorimetric (DAB).

Detection of human ACTN4 by immunocytochemistry. Sample: Paraformaldahyde treated HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (20 µg/ml). Detection: fluorescence).
References
ReferencesYamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg. 39, 61.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlPC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (20 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein
Clone4D10
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CB1024-100UGCN 04055977228694

Documentation

Anti-ACTN4 Mouse mAb (4D10) MSDS

Title

Safety Data Sheet (SDS) 

Anti-ACTN4 Mouse mAb (4D10) Certificates of Analysis

TitleLot Number
CB1024

References

Reference overview
Yamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg. 39, 61.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision29-October-2010 RFH
SynonymsAnti-Actinin α4
ApplicationELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (20 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of rat ACTN4 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse ACTN4 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

Detection of Human ACTN4 by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Priamry antibody: Anti-ACTN4 Mouse mAb (AD10) (Cat. No. CB1024) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human ACTN4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (3 µg/ml). Detection: colorimetric (DAB).

Detection of human ACTN4 by immunocytochemistry. Sample: Paraformaldahyde treated HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-ACTN4 Mouse mAb (4D10) (Cat. No. CB1024) (20 µg/ml). Detection: fluorescence).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~90-100 kDa ACTN4 protein.
BackgroundAlpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein
Clone4D10
IsotypeIgG2a
Specieshuman, mouse, rat
Positive controlPC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesYamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg. 39, 61.