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AB9744 Anti-14-3-3 τ (tau) Antibody

Detect 14-3-3 τ (tau) using this Anti-14-3-3 τ (tau) Antibody validated for use in ELISA, WB, IH.

Anti-14-3-3 τ (tau) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: YWHAQ, HS1, 14-3-3, 1C5 UniProt Number: P27348
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AB9744
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, Ch, H, M, R, Rb, ShELISA, WB, IHCRbSerumPolyclonal Antibody
Description
Catalogue NumberAB9744
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-14-3-3 τ (tau) Antibody
Background InformationMammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's.

The 14-3-3 tau antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer's and Parkinson's Disease.
References
Product Information
FormatSerum
PresentationLiquid.
Quality LevelMQ100
Applications
ApplicationDetect 14-3-3 τ (tau) using this Anti-14-3-3 τ (tau) Antibody validated for use in ELISA, WB, IH.
Key Applications
  • ELISA
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern blot: 1:3,000 using ECL.
Immunohistochemistry: 1:30-1:60 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes.
ELISA
Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenSynthetic peptide from the N-terminal area of sheep 14-3-3 tau.
HostRabbit
Specificity14-3-3 tau (theta). By Western blot the antibody recognizes the ~30 kDa protein.
Species Reactivity
  • Bovine
  • Chicken
  • Human
  • Mouse
  • Rat
  • Rabbit
  • Sheep
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.
Gene Symbol
  • YWHAQ
  • HS1
  • 14-3-3
  • 1C5
Purification MethodSerum
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P27348 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
SIZE: 245 amino acids; 27764 Da
SUBUNIT: Interacts with PCTK1 (By similarity). Homodimer. Interacts with SSH1.
SUBCELLULAR LOCATION: Cytoplasm. Note=In neurons, axonally transported to the nerve terminals.
TISSUE SPECIFICITY: Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
SIMILARITY: SwissProt: P27348 ## Belongs to the 14-3-3 family.
Molecular Weight30 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB9744 04053252509254

Documentation

Anti-14-3-3 τ (tau) Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-14-3-3 τ (tau) Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-14-3-3 tau - 3277978 3277978
RABBIT ANTI-14-3-3 tau POLYCLONAL ANTIBODY 2476711

References

Reference overviewPub Med ID
Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1
Chen, Hung-Kai, et al
Cell, 113:457-68 (2003)  2003

12757707 12757707
Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain
Baxter, H C, et al
Neuroscience, 109:5-14 (2002)  2002

11784696 11784696
Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation
Wheeler-Jones, C P, et al
Biochem J, 315 ( Pt 1):41-7 (1996)  1996

8670130 8670130