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SCC153 9HTEo- Human Tracheal Epithelial Cell Line

9HTEo- human tracheal epithelial cell line is a useful model for the study of epithelial ion transport, secretion and biochemistry.

9HTEo- Human Tracheal Epithelial Cell Line MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture, Cell Based Assays
SCC153
≥1X10⁶ cells/vial   
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
CULT, Cell Based Assays
Description
Catalogue NumberSCC153
Description9HTEo- Human Tracheal Epithelial Cell Line
Alternate Names
  • 9/HTEo-
  • 9/HTEo
  • 9HTE
Background Information9HTEo- is a human tracheal epithelial cell line that was immortalized with the origin-of-replication defective SV40 plasmid (pSVori-). The cell line retains characteristic features of normal differentiated tracheal epithelial cells including a cobblestone morphology, cytokeratin expression and the ability to form tight junctions. 9HTEo- cells contain the wild-type cystic fibrosis transmembrane conductance regulator (WT-CFTR) and exhibits functional cAMP-dependent chloride ion transport and calcium ion-dependent chloride transport (1,2).

9HTEo- is a useful model for the study of epithelial ion transport, secretion and biochemistry and frequently used as a wild-type control for human CF tracheobronchial epithelial cells such as CFBE41o- (Cat. No. SCC151) and CFTE29o- (Cat. No. SCC162).

References:
1. Gruenert DC, Basbaum CB, Welsh MJ, Li M, Finkbeiner WE, Nadel JA. (1988) Characterization of human tracheal epithelial cells transformed by an origin-defective simian virus. Proc. Natl. Acad. Sci. USA 85: 5951-5955.
2. Cozens AL, Yezzi MJ, Chin L, Simon EM, Finkbeiner WE, Wagner JA, Gruenert DC. (1992) Characterization of immortal cystic fibrosis tracheobronchial gland epithelial cells. Proc. Natl. Acad. Sci. USA 89(11): 5171-5175.
References
Product Information
Applications
Application9HTEo- human tracheal epithelial cell line is a useful model for the study of epithelial ion transport, secretion and biochemistry.
Key Applications
  • Cell Culture
  • Cell Based Assays
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Epithelial Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCC153 04054839480447