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69015 pET-32a(+) DNA - Novagen

Novagen's pET-32a(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein.

pET-32a(+) DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69015
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69015-3CN Plastic ampoule 10 μg
Description
OverviewThe pET-32a(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein (1). Cloning sites are available for producing fusion proteins also containing cleavable His•Tag® and S•Tag™ sequences for detection and purification. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB122VM. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number69015
Brand Family Novagen®
References
References1. LaVallie, E.R., DiBlasio, E.A., Kovacic, S., Grant, K.L., Schendel, P.F. and McCoy, J.M. (1993) Bio/Technology 11, 187–193.
Product Information
Vector familypET
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69015-3CN 07790788052430

Documentation

pET-32a(+) DNA - Novagen Certificates of Analysis

TitleLot Number
69015

References

Reference overview
1. LaVallie, E.R., DiBlasio, E.A., Kovacic, S., Grant, K.L., Schendel, P.F. and McCoy, J.M. (1993) Bio/Technology 11, 187–193.

Brochure

Title
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

Citations

Title
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  • Adam Joelsson, A., et al. 2007. Applied and Enviornmental Microbiology 73, 3742.
  • Wong, H.L., et al. 2007. Plant Cell 19, 4022.
  • Gyan, S., et al. 2006. Journal of Bacteriology 188, 7062.
  • Masin, M., et al. 2006. Journal of Biological Chemistry 281, 4100.
  • Oertelt, S., et al. 2006. Journal of Immunology 177, 1655.
  • Smith R.M., and Williams S.B., 2006. Proceedings of the National Academy of Sciences (USA) 103, 8564.
  • Suzuki, H., et al. 2006. Journal of Biological Chemistry 281, 30251.
  • Vercauteren, K., et al. 2006. Molecular and Cellular Biology 26, 7409.
  • Anjard, C., and Loomis, W.F., 2005. Procedings of the National Academy of Science 102, 7607.
  • Bracken, C.P., et al. 2005. Journal of Biological Chemistry 280, 14240.
  • Don J. Davidson, D.J., et al. 2005. Cancer Research 65, 4663.
  • Dufour, E.K., et al. 2005. Protein Science 14, 303.
  • Hoffmeister, M., et al. 2005. Journal of Biological Chemistry 280, 4329.
  • Hymowitz, S.G., et al. 2005. Journal of Biological Chemistry 280, 7218.
  • Kani, K., et al. 2005. Journal of Biological Chemistry 280, 8238.
  • Katou, S., et al. 2005. Journal of Biological Chemistry 280, 39569.
  • Lara, M.V., et al. 2005. Plant and Cell Physiology 46, 997.
  • Lo, K.W., et al. 2005. Journal of Biological Chemistry 280, 8172.
  • Ran X., and Song J., 2005. Journal of Biological Chemistry 280, 19205.
  • Schilling, O., et al. 2005. Journal of Biological Chemistry 280, 17857.
  • Takahashi-Terada, A., et al. 2005. Journal of Biological Chemistry 280, 11798.
  • Yoshida S., and Parniske M., 2005. Journal of Biological Chemistry 280, 9203.
  • Holland M.C.H., and Lambris J.D., 2004. Journal of Immunology 172, 349.
  • Ichinoe, A., et al. 2004. i>Nucleic Acids Research 32, 477.
  • Mancini, E.J., et al. 2004.Acta Crystallographica D60, 588.
  • Raschle,M., et al. 2004. Journal of Biological Chemistry 279, 51973.
  • Trabbic-Carlson, K., et al. 2004. Protein Engineering Design and Selection 17, 57.
  • Marina V. Backer, M.V., et al. 2003. Protein Expression and Purification 26, 455.
  • Perrin, M.H., et al. 2003. Journal of Biological Chemistry 278, 15595.
  • Rosenblum, M.G., et al. 2003. Cancer Research 63, 3995.
  • Lobel, L., et al. 2002. Protein Expression and Purification 25, 124.
  • Koffa, M.D., et al. 2001. European Molecular Biology Organization Journal 20, 5769.
  • Lauber, T., et al. 2001. Protein Expression and Purification 22, 108.
  • Lobel, L.I., et al. 2001. Endocrine 14, 205.
  • Huang, J.D., et al. 1999. Nature 397, 267.
  • Becker, S., et al. 1998. nature 394, 145.
  • Xu, G.L., and Bestor T.H., 1997. Nature Genetics 17, 376.
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    User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB122VM pET-32a-c(+) Vector Map

    Vector Sequence

    Title
    pET-32a(+) Vector Sequence